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LabAssay Creatinine Technical Information and Data
Performance
| Sample | Serum, Plasma, Urine |
|---|---|
| Target animals | Mouse, Rat, Cat, Dog |
| Standard curve range | 2.5 - 10mg/mL (mg/100 mL) |
| Measurement time | Approx. 40 min. |
| Amount of sample | 50 μL |
| Measurement wavelength | 520 nm |
Standard curve
Procedure
Perform the assay in the wells according to the following table scheme.
| Test | Standard | Blank | |
|---|---|---|---|
| First operations should be performed in the sample tubes. | |||
| Sample | Sample 50 μL |
Standard solution 50 μL |
Water 50 μL |
| Deproteinizing reagent | 300 μL | 300 μL | 300 μL |
| Mix well, and stand at room temperature for 10 min. Centrifuge over 2500 rpm for 10 min. Subsequent operations should be performed in the wells. |
|||
| Supernatant | 100 μL | 100 μL | 100 μL |
| Stand at 25 ℃ - 30 ℃ | |||
| Picric Acid Reagent | 50 μL | 50 μL | 50 μL |
| 0.75 mol/L Sodium Hydroxide Solution | 50 μL | 50 μL | 50 μL |
| Stand at 25°C - 30°C for 20 min. Measure the absorbance of the test sample and standard solution at 520 nm with the blank solution as the control within the next 30 min. |
|||
Data
Spike-and-Recovery Test
Spike and Recovery Test of Creatinine, a known amount of standard is spiked into in mouse and rat sample n=2.
| Rat Sample | Spike (mg/dL) | Mean (mg/dL) | Recovery (mg/dL) | Recovery (%) |
|---|---|---|---|---|
| Serum | - | 2.86 | - | - |
| 3.27 | 5.61 | 2.75 | 84.1 | |
| 5.60 | 8.13 | 5.27 | 94.1 | |
| 7.35 | 9.34 | 6.48 | 88.2 | |
| Plasma (EDTA) |
- | 2.78 | - | - |
| 2.79 | 5.22 | 2.44 | 87.5 | |
| 4.78 | 8.3 | 5.52 | 115 | |
| 6.27 | 9.22 | 6.44 | 103 | |
| Plasma (Heparin) |
- | 2.88 | - | - |
| 3.17 | 5.53 | 2.65 | 83.6 | |
| 5.43 | 8.25 | 5.37 | 98.9 | |
| 7.13 | 9.27 | 6.39 | 89.6 |
| Mouse Sample | Spike (mg/dL) | Mean (mg/dL) | Recovery (mg/dL) | Recovery (%) |
|---|---|---|---|---|
| Serum | - | 2.68 | - | - |
| 3.00 | 5.90 | 3.22 | 107 | |
| 5.14 | 8.08 | 5.40 | 105 | |
| 6.74 | 8.90 | 6.22 | 92.3 | |
| Plasma (EDTA) |
- | 3.14 | - | - |
| 2.71 | 5.63 | 2.49 | 91.9 | |
| 4.65 | 7.74 | 4.60 | 98.9 | |
| 6.10 | 8.25 | 5.11 | 83.8 | |
| Plasma (Heparin) |
- | 3.01 | - | - |
| 2.85 | 5.48 | 2.47 | 86.7 | |
| 4.88 | 7.74 | 4.73 | 96.9 | |
| 6.41 | 8.60 | 5.59 | 87.2 |
Linearity-of-Dilution Test
Rat sample
Mouse sample
Example of measurement
| Normal Rat serum, Sprague Dawley, male | |||||
|---|---|---|---|---|---|
| No. | Measured (mg/dL) | mean | SD | CV (%) | |
| 1 | 0.553 | 0.546 | 0.550 | 0.0049 | 0.90 |
| 2 | 0.716 | 0.824 | 0.770 | 0.076 | 9.9 |
| 3 | 1.12 | 1.26 | 1.19 | 0.095 | 8.0 |
| 4 | 1.22 | 1.29 | 1.25 | 0.050 | 4.0 |
| 5 | 1.04 | 0.994 | 1.01 | 0.029 | 2.9 |
| Normal Rat plasma K2EDTA, Sprague Dawley, male | |||||
|---|---|---|---|---|---|
| No. | Measured (mg/dL) | mean | SD | CV (%) | |
| 1 | 0.319 | 0.288 | 0.304 | 0.022 | 7.2 |
| 2 | 0.638 | 0.627 | 0.633 | 0.0078 | 1.2 |
| 3 | 0.824 | 0.871 | 0.848 | 0.033 | 3.9 |
| 4 | 0.661 | 0.681 | 0.671 | 0.014 | 2.1 |
| 5 | 0.205 | 0.233 | 0.219 | 0.020 | 9.0 |
| Normal Mouse serum, CD-1(ICR), male | |||||
|---|---|---|---|---|---|
| No. | Measured (mg/dL) | mean | SD | CV (%) | |
| 1 | 0.199 | 0.205 | 0.202 | 0.0042 | 2.1 |
| 2 | 0.492 | 0.507 | 0.500 | 0.011 | 2.1 |
| 3 | 0.734 | 0.722 | 0.728 | 0.0085 | 1.2 |
| 4 | 0.641 | 0.716 | 0.679 | 0.053 | 7.8 |
| 5 | 0.192 | 0.219 | 0.206 | 0.019 | 9.3 |
| Normal Mouse plasma K2EDTA, CD-1(ICR), male | |||||
|---|---|---|---|---|---|
| No. | Measured (mg/dL) | mean | SD | CV (%) | |
| 1 | 0.0950 | 0.103 | 0.0990 | 0.0057 | 5.7 |
| 2 | 0.607 | 0.559 | 0.583 | 0.034 | 5.8 |
| 3 | 0.556 | 0.528 | 0.542 | 0.020 | 3.7 |
| 4 | 0.490 | 0.546 | 0.518 | 0.040 | 7.6 |
| 5 | 0.113 | 0.110 | 0.112 | 0.0021 | 1.9 |
| Normal Canine serum, Beagle, male | ||||||
|---|---|---|---|---|---|---|
| No. | Measured (mg/dL) | mean | SD | CV (%) | Age (years) | |
| 1 | 1.67 | 1.48 | 1.57 | 0.13 | 8.4 | 4 |
| 2 | 1.16 | 1.07 | 1.11 | 0.063 | 5.7 | 6 |
| 3 | 1.54 | 1.41 | 1.48 | 0.092 | 6.2 | 6 |
| 4 | 1.58 | 1.40 | 1.49 | 0.13 | 8.9 | 11 |
| 5 | 1.46 | 1.49 | 1.47 | 0.023 | 1.6 | 12 |
| 6 | 1.17 | 1.20 | 1.19 | 0.027 | 2.3 | 6 |
| 7 | 1.16 | 1.20 | 1.18 | 0.027 | 2.3 | 10 |
| 8 | 1.23 | 1.30 | 1.27 | 0.047 | 3.7 | 4 |
| Normal Canine plasma EDTA, Beagle, male | ||||||
|---|---|---|---|---|---|---|
| No. | Measured (mg/dL) | mean | SD | CV (%) | Age (years) | |
| 1 | 1.48 | 1.45 | 1.46 | 0.021 | 1.5 | 4 |
| 2 | 1.21 | 1.18 | 1.20 | 0.024 | 2.0 | 6 |
| 3 | 1.33 | 1.22 | 1.27 | 0.071 | 5.6 | 10 |
| 4 | 1.56 | 1.49 | 1.53 | 0.048 | 3.2 | 12 |
| 5 | 1.31 | 1.29 | 1.30 | 0.0099 | 0.76 | 6 |
| 6 | 1.04 | 1.04 | 1.04 | 0.0028 | 0.27 | 11 |
| 7 | 1.14 | 1.16 | 1.15 | 0.013 | 1.2 | 10 |
| 8 | 1.26 | 1.29 | 1.28 | 0.019 | 1.5 | 12 |
| Normal Feline serum, Short hair, male | ||||||
|---|---|---|---|---|---|---|
| No. | Measured (mg/dL) | mean | SD | CV (%) | Age (years) | |
| 1 | 1.86 | 1.78 | 1.82 | 0.060 | 3.3 | 3.0 |
| 2 | 1.58 | 1.53 | 1.55 | 0.034 | 2.2 | 3.0 |
| 3 | 1.81 | 1.68 | 1.75 | 0.092 | 5.3 | 6.0 |
| 4 | 2.17 | 1.95 | 2.06 | 0.16 | 7.6 | 4.0 |
| 5 | 1.88 | 1.92 | 1.90 | 0.030 | 1.6 | 6.0 |
| 6 | 1.89 | 1.86 | 1.87 | 0.017 | 0.91 | 6.0 |
| 7 | 1.71 | 1.76 | 1.73 | 0.040 | 2.3 | 1.0 |
| 8 | 1.48 | 1.56 | 1.52 | 0.055 | 3.6 | 1.0 |
| Normal Feline plasma EDTA, Short hair, male | ||||||
|---|---|---|---|---|---|---|
| No. | Measured (mg/dL) | mean | SD | CV (%) | Age (years) | |
| 1 | 1.63 | 1.61 | 1.62 | 0.011 | 0.65 | 0.5 |
| 2 | 1.41 | 1.36 | 1.39 | 0.038 | 2.8 | 1.0 |
| 3 | 1.78 | 1.62 | 1.70 | 0.11 | 6.7 | 3.0 |
| 4 | 1.97 | 1.76 | 1.87 | 0.15 | 7.9 | 0.5 |
| 5 | 1.66 | 1.67 | 1.66 | 0.0085 | 0.51 | 3.0 |
| 6 | 1.28 | 1.29 | 1.29 | 0.0028 | 0.22 | 3.0 |
| 7 | 1.26 | 1.30 | 1.28 | 0.029 | 2.3 | 0.5 |
| 8 | 1.60 | 1.71 | 1.65 | 0.080 | 4.8 | 1.0 |
