We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Human/Rat β Amyloid (42) ELISA Kit Wako, High Sensitive Data
Performance
Standard curve
Normal Human Plasma (A-H) were measured using the ELISA Kit Wako Ⅱand the conventional kit. The obtained results were equivalent for the two kits.
Spike-and-recovery-test
Standard curves were prepared using the standard solution included in the kit.
Dilution linearity test
Normal Human Plasma (A, C, E) were diluted 2-16 folds to determine the dilution linearity. Human/Rat Aβ exhibited linear dilution.
Specificity test
| Sample | Cross-reactivity (%) |
|---|---|
| Human Aβ (1-40) | ≦ 0.1 |
| Human Aβ (1-42) | 100.0 |
| Human Aβ (1-43) | 12.7 |
| Rat (Mouse) Aβ (1-40) | ≦ 0.1 |
| Rat (Mouse) Aβ (1-42) | 156.0 |
Application Data
Measurements in human and mouse plasma
Blood samples collected in evacuated blood collection tubes with K2EDTA were centrifuged for 15 min. at 5,000 x g and 4°C. Plasma was isolated and kept in -80°C until use.
The plasma samples were diluted 4-fold using the standard dilution solution contained in the kit prior to the measurements.
(※Human β Amyloid (1-40) ELISA Kit Wako II (Catalog ID 298-64601) and Human/Rat β Amyloid (40) ELISA Kit Wako II (Catalog ID 294-64701) were used for the measurements of Aβ [1-40] and Aβ [x-40]).
Measurements in the mouse brain
The brain hemisphere of a 12-week-old APP transgenic mouse (J20) was homogenized with 2 mL Tris-buffered Saline and kept in -20°C until use.
The extract was diluted 2-fold using the standard diluent solution contained in the kit prior to the measurements.
A small amount of Aβ was detected in samples from both transgenic (tg) and wild-type (wt) mice.
(Data provided by: Drs. Iwatsubo and Hashimoto, Department of Neuropathology, Graduate School of Medicine, University of Tokyo)
