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Human/Rat β Amyloid (40) ELISA Kit Wako II
for Immunochemistry
These ELISA kits are detection kits that achieve more stable antigen-antibody reactions by using F(ab')2 fragment of the labeled antibody BA27, which recognizes the C-terminal of beta Amyloid 40, while the nonspecific binding is remaining to be reduced. Therefore, the stability in washing solution is improved. In the Human/Rat kit, background is reduced.
*1: beta Amyloid (x-40) is beta Amyloid peptide modified or cleaved at the N-terminal.
Aimed at stable Antigen-Antibody Reaction
- Human beta Amyloid (1-40) ELISA Kit Wako II *2(Wako Catalog #298-64601; 96 tests)
- Human/Rat beta Amyloid (40) ELISA Kit Wako II *3 (Wako Catalog #294-64701; 96 tests)
This product is for research use only. Do not administer it to human.
Manufacturer: |
FUJIFILM Wako Pure Chemical Corporation |
GHS: | ![]() ![]() |
Features
Senile plaques are found specifically in the brain of patients with Alzheimer disease. The main component of senile plaque is β amyloid peptide (Aβ), which consists of 40 or 42 (43) amino acids. Aβ is therefore considered a potential marker for Alzheimer’s disease. As such, a method is needed to detect the level of Aβ with high sensitivity.
The kit is used to perform a sandwich ELISA assay with a highly specific monoclonal antibody developed by Takeda Pharmaceutical Company Ltd.. It enables quantification of human or rat (mouse) Aβ (40) with truncated or modified N-terminus from the brain tissue, culture supernatant, cerebrospinal fluid, as well as from the plasma which was conventionally challenging to quantify.
The F(ab)’2 fragment of the antibody (BA27) is used to recognize the C-terminal of Aβ40. As a result, non-specific binding is minimized and the stability in terms of antigen-antibody reaction has improved compared with the conventional kit*. Thus, the antibody has an improved stability even during the washing step.
Improvements from the conventional kit
- Improved stability of antigen-antibody reaction as a result of the use of F(ab)’2 fragment of the antibody (BA27) to recognize the C-terminal.
- Lower background signal for human/rats (about 1/3 of the conventional kit).
*Human βAamyloid(40) ELISA Kit Wako (Code No. 294-62501)
- Achieve more stable antigen-antibody reaction by using BA27 (F(ab')2-HRP)
- Human/Rat beta Amyloid (40) ELISA Kit Wako II ... Background is reduced by 60% compared to "Human/Rat beta Amyloid (40) ELISA Kit" (#294-62501)
Kit component
For 96 Tests
Antibody (BNT77)-coated Microtiter Plate (Anti Human Aβ [1-16] MoAb [Clone No. BNT77]) | 1 plate |
Standard Solution (Human βAmyloid [1-40], 100 pmol/L) | 2 mL x 2 |
Standard Diluent Solution | 30 mL x 1 |
Wash Solution (20x) | 50 mL x 1 |
HRP-conjugated Antibody (BA27) Solution (Anti Human Aβ [1-40] MoAb [Clone No. BA27] F(ab’)2-HRP) | 12 mL x 1 |
TMB Solution | 12 mL x 1 |
Stop Solution | 12 mL x 1 |
Plate Seal | 3 sheets |
The product information is shown on the page 7 of Wako Product Update No. 17.
Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific
- For research use only. Not for use in diagnostic procedures.
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