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Human β Amyloid (1-42) ELISA Kit Wako, High Sensitive Data
Performance
Standard curve
Standard curves were prepared using the standard solution included in the kit. The curves resembled a straight line between 0.1-20.0 pmol/L.
Spike-and-recovery test
Standard curves were prepared using the standard solution included in the kit. The curves resembled a straight line within the range between 0.1-20.0 pmol/L.
※2-hr reaction with the HRP-conjugated antibody
Dilution linearity test
Normal Human Plasma (A, C, E) were diluted 2-16 folds to determine the dilution linearity.
Specificity test
| Sample | Cross-reactivity (%) |
|---|---|
| Human Aβ (1-40) | ≦ 0.1 |
| Human Aβ (1-42) | 100.0 |
| Human Aβ (1-43) | 13.5 |
| Rat (Mouse) Aβ (1-40) | ≦ 0.1 |
| Rat (Mouse) Aβ (1-42) | 0.5 |
Application Data
Measurements in human and mouse plasma
Blood samples collected in evacuated blood collection tubes with K2EDTA were centrifuged for 15 min. at 5,000 x g and 4°C. Plasma was isolated and kept in -80°C until use.
The plasma samples were diluted 4-fold using the standard diluent solution contained in the kit prior to the measurements.
(※Human β Amyloid(1-40) ELISA Kit Wako II (Catalog ID 298-64601) and Human/Rat β Amyloid(40) ELISA Kit Wako II (Catalog ID 294-64701) were used for the measurements of Aβ [1-40] and Aβ [x-40]).
(Data provided by: Drs. Iwatsubo and Hashimoto, Department of Neuropathology, Graduate School of Medicine, University of Tokyo)
