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High Molecular Amyloid β Oligomer ELISA Kit Wako Ver.2 Technical Information and Data
Assay principal
This ELISA kit is designed to measure ≧ 9-mer Aβ oligomer specifically by using same Aβ antibody (BAN50) for capture and detection3)4). As a standard, a 16-mer MAP peptide with 16 epitopes of anti Aβ antibody (BAN50) is used4).


3) Fukumoto, H., et al.: FASEB J., 24, 2716(2010).
4) Kasai, T., et al.: Biochem. Biophys. Res. Commun., 422, 3, 375(2012).
Specificity for Aβ oligomers in the ELISA
Aβ monomers were incubated the solution at 4℃ overnight in order to synthesize various size of Aβ oligomers. They were separated Fraction A and Fraction B by ultrafiltration. The fractions were analyzed by western blotting and the Aβ oligomer ELISA.


Western blotting using Aβ antibody(BAN50)


Aβ oligomer ELISA


A strong signal was exhibited in the fraction A containing 9-mer and more (> 30 kDa), whereas few signal was detected in fraction B containing 8-mer and smaller (< 30 kDa).
Example of measurements
Example of measurement 1 - spiked recovery test and dilution linearity
Human plasma | Not spiked | +0.5pM Std. | +0.1pM Std. | +5pM Std. | +30pM Std. | ||||
---|---|---|---|---|---|---|---|---|---|
Assay value | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | |
1.85pM | 2.36pM | 102% | 2.85pM | 100% | 7.11pM | 105% | 31.2pM | 97.8% | |
Human serum | Not spiked | +0.5pM Std. | +0.1pM Std. | +5pM Std. | +30pM Std. | ||||
Assay value | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | |
1.58pM | 2.07pM | 98% | 2.59pM | 101% | 6.64pM | 101% | 31.9pM | 101% |
Human CSF1 | Not spiked | +3pM Std. | +6pM Std. | +15pM Std. | |||
---|---|---|---|---|---|---|---|
Assay value | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | |
5.16pM | 8.01pM | 95% | 10.6pM | 91% | 18.1pM | 86% | |
Human CSF2 | Not spiked | +3pM Std. | +6pM Std. | +15pM Std. | |||
Assay value | Assay value | Recovery | Assay value | Recovery | Assay value | Recovery | |
29.1pM | 38.8pM | 97% | 44.0pM | 99.3% | 59.9pM | 88% |


Example of measurement 2 - CSF sample
Cerebrospinal fluids(CSF) of patients with Alzheimer disease (AD) and Mild Cognitive Impairment (MCI), non-dementia patients(control), were measured with our ELISA kit and competitor’s kit.
Human Serum
Age | Sex | MMSE※3 | CSF Aβ oligomer(pM) |
|
---|---|---|---|---|
Control n=5 |
69 | M | 30 | 2.37 |
60 | F | 30 | 1.65 | |
59 | M | 30 | 2.82 | |
46 | M | 28 | 2.24 | |
60 | F | 29 | 1.34 | |
MCI n=5 |
69 | F | 24 | 3.6 |
76 | M | 23 | 2.62 | |
82 | M | 26 | 2.33 | |
69 | F | 23 | 2.67 | |
73 | F | 25 | 2.34 | |
AD n=8 |
79 | F | 10 | 3.56 |
88 | M | 24 | 3.13 | |
62 | M | 16 | 4.13 | |
78 | M | 13 | 3.95 | |
63 | M | 14 | 3.88 | |
74 | F | 16 | 3.25 | |
76 | M | 19 | 3.69 | |
84 | M | 16 | 4.34 |
***MMSE score:diagnosis Inquiry test score of cognitive function
30-28 Normal
27-24 Suspected of mild cognitive impairment
≦ 23 Suspected of dementia
Human Plasma


- There was a significant difference between Control, MCI and AD.
- High molecular Aβ oligomer correlated with MMSE score.