This product is highly purified exosomes isolated from a cell culture supernatant of COLO201 cells through PS affinity method, using Tim4 protein which specifically binds phosphatidylserine (PS) displayed on the surface of exosomes.

• High purity
• Long-term stability
• Ready-to-use liquid format
• CD9, CD63 and CD81 positive

• Physical State：Liquid
• Constituent：Exosomes, TBS, EDTA, Polymer as a stabilizer
• Concentration：10 μg/mL*

*The value is corrected protein concentration by a CD63 signal.

10 ng of “Exosomes, from COLO201 cells, purified” was loaded on each well and SDS-PAGE was performed under non-reducing conditions. Then, canonical exosome surface markers such as CD9, CD63 and CD81 were detected using anti-CD9, anti-CD63 and anti-CD81 antibodies.

Primary antibodies

• Anti CD9, Monoclonal Antibody (1K) (Product code: 014-27763)
• Anti CD63, Monoclonal Antibody (3-13) (Product code: 012-27063)
• Anti CD81, Monoclonal Antibody (17B1) (Product code: 011-27773)

Secondary antibody

• HRP conjugated anti-Mouse IgG

CD9, CD63 and CD81 are highly expressed on “Exosomes, from COLO201 cells, purified” 

Next, 10 ng of “Exosomes, from COLO201 cells, purified” and five on-market Exosomes were loaded on each well and SDS-PAGE was performed under non-reducing conditions. Then, CD63 was detected using anti-CD63 antibodies.

Lane No.

① “Exosomes, from COLO201 cells, purified”
② Competitor 1: Exosomes, from A549 cells
③ Competitor 2: Exosomes, from HEK293 cells
④ Competitor 3: Exosomes, from A549 cells
⑤ Competitor 4: Exosomes, from COLO1 cells
⑥ Competitor 5: Exosomes, from HCT116 cells

Primary antibody

Anti CD63, Monoclonal Antibody (3-13) (Product code: 012-27063)

Secondary antibody

HRP conjugated anti-Mouse IgG

Our “Exosomes, from COLO201 cells, purified” only showed a CD63 signal compared to five on-market Exosomes in detecting the presence of CD63.

Comparison of particle size analysis between “Exosomes, from COLO201 cells, purified” and Exosomes from Competitor 1 using NanoSight.

“Exosomes, from COLO201 cells, purified” have expected particle size distribution.

Structure of “Exosomes, from COLO201 cells, purified” which have been frozen at -20℃ and thawed at room temperature 15 times was observed by an electron microscope.

“Exosomes, from COLO201 cells, purified” was maintained their structure even they have undergone 15 times freeze-thaw cycles.