Q: What kinds of organoids have been produced?

A: Organoids for brain, gut, and kidney.

Q: What is the impact of CEPT Cocktail on 3D culturing?

A: Here is the impact.

• Optimization of cell aggregation and survival
• Improvement of reproducibility
• Stress suppression during cell differentiation
• Improvement of three-dimensional structure and self-organization

Q: At what time is is the CEPT Cocktail added to the solution during passaging of iPSC?

A: During the first 24 hours of passaging.

Q: At what time is the CEPT Cocktail added to the solution during freezing and thawing of iPSC?

A: CEPT Cocktail should be added to the cryopreservation solution and frozen.
After thawing and cell seeding, cells should be cultured in medium with CEPT Cocktail for the first 24 hours.

Q: What should I check if I want more information?

A: Please review the following papers:

• Chen, Y. et al. : Nat. Methods, 18(5), 528 (2021).
  A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells.
• Tristan, C.A. et al. : Stem Cell Reports, 16(12), 3076 (2021).
  Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells.
• Tristan, C.A. et al. : Nat. Protoc., 18(1), 58 (2022).
  Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail.
• Deng, T. et al. : Stem Cell Reports, 18(4), 1030 (2023).
  Scalable generation of sensory neurons from human pluripotent stem cells.
• Takeshi, W. et al. : Regen. Med., 18(3), 219 (2023).
  International evaluation study of a highly efficient culture assay for detection of residual human pluripotent stem cells in cell therapies.

Q: Is the solution animal-free?

A: Yes it is. Please contact us if you wish to issue a certificate.