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CTGrade GMP rh PDGF-AA is a recombinant human protein that is produced from E. coli and is designed to support preclinical and clinical research, as well as commercial applications, and offers:
High biological activity verified by a relevant bioactivity assay
Low endotoxin levels
≥ 97% purity
High lot-to-lot consistency
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
PDGF (platelet-derived growth factor)-AA serves as a crucial controller of cell growth, proliferation, and angiogenesis, and its synthesis is stimulated by signaling molecules, such as IL-1, IL-6, TNF-α, TGF-β, and EGF.
Differentiates neural progenitor cells derived from human pluripotent stem cells into oligodendrocyte precursor cells
Acts as a growth hormone that induces cell division in mesenchymal lineage cells like smooth muscle and glial cells
Releases from the alpha-granules of platelets when platelets adhere to injured tissue
Binds to cell surface receptor tyrosine kinases, specifically PDGFR-α and PDGFR-β
For the development of advanced cell therapies, FUJIFILM Irvine Scientific is your one-stop-shop for the high-performing recombinant proteins required for expansion, proliferation, differentiation, and activation of immune and stem cells. Our CTGrade GMP recombinant proteins can be purchased separately or as a bundle with one of our high-performing PRIME-XV or classical media for a cost-effective solution.
The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade GMP products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
SDS-PAGE Showing a Single Prominent Band at 32 kDa
Figure 1. CTGrade GMP rh PDGF-AA (1 μg) was resolved with SDS-PAGE under reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 32 kDa (R).
Cell-Based Proliferation Assay
Figure 2. The biological activity of CTGrade GMP rh PDGF-AA was determined in a NR6R/3T3 cell-based proliferation assay.
CTGrade GMP rh PDGF-AA is lyophilized from a 0.2 μm filtered solution containing 0.1% trifluoroacetic acid
Protein Purity
≥ 97% determined by reducing and non-reducing SDS-PAGE analysis
Endotoxin
≤ 0.01 EU/μg using USP <85> / EP 2.6.14
Bioactivity
ED50 is determined by the dose-dependent proliferation of NR6R-3T3 cells. The ED50 is typically less than 50 ng/mL. Activity is > 1.0 x 106 U/mg when calibrated against the internal standard PDGF-AA measured in cell proliferation assay using NR6R-3T3 cells.
Quality
Carrier-free and no animal- or human-derived materials were used during manufacturing
Specifications
Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
