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CTGF (Full+N-terminal region) ELISA Kit Wako
About Sample Preparation
Q: What anticoagulant should be used for blood samples?
A: The literature referenced below indicates that the values for N-terminal CTGF are higher when using heparin plasma than when using EDTA or citrate plasma. Heparin should be avoided as an anticoagulant.
Reference: Miyazaki, O. et al.: Ann. Clin. Biochem., 47, 205(2010).
About the Kit
Q: Are the antibodies used in this kit available for purchase?
A: Anti CTGF Module 1 monoclonal antibody (30D2) (Product Number: 018-27423)
The detection antibody is not sold separately. The antibody for Module 2 (Product Number: 015-27433) is available, but the clone is different from the one used for the antibody in this kit.
Q: How is the standard quantified?
A: The standard is prepared and quantified according to the following literature.
Reference: Miyazaki, O. et al.: Ann. Clin. Biochem., 47, 205(2010).
About Kit Usage
Q: What reagents, instruments, and equipment are required for the assay using this kit?
A: The following are required.
- Purified water（distilled water）
- Test tubes for dilution of standard solution and samples
- Glass utensils for dilution of Wash Solution (graduated cylinder, beaker)
- Pipettes with disposable tips (one capable of pipetting 10μL of liquid accurately and one capable of pipetting 200 to 500 μL)
- Repeater pipette, capable of repeatedly dispensing 100μL
- Water-absorbent material such as a paper towel (to remove any solution remaining on the plate after washing)
- Vortex-type mixer
- Microplate shaker (range approx. 500 to 800 rpm)
- Automatic washer for 96-well plate (if available) or washing bottle
- Microplate reader capable of measuring at 450 ± 10 nm, with the correction wavelength set at 600 to 650 nm
- Software for data analysis
Q: Is it necessary to always use this kit with the CTGF (Full) ELISA Kit Wako (Catalog ID: 290-84701)?
A: Both this kit and the CTGF (Full) ELISA Kit Wako are required only when measuring the N-terminal CTGF (Module 1 - 2).
Q: How many samples can be measured using the 96 tests?
A: Since 16 wells are needed for standards (n = 2), 80 samples (n = 1) or 40 samples (n = 2) can be measured.
Q: Can the kit be divided for use?
A: Yes, it can. The plate can be cut at every eight-well boundary, and lots are available for standard use. It should be noted that fewer samples can be analyzed when the kit is divided for use, because a calibration curve needs to be drawn each time.
