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CD9/63/81-Capture Human Exosome ELISA Kit (Streptavidin HRP) Technical Information
Application of the PS affinity method to ELISA
- Specific to human extracellular vesicles.
- Direct analysis from cell culture sup. or blood samples. No need for isolation.
- Qualitative and quantitative analysis.
Note: Purified extracellular vesicle standard is required for quantification. - Simple operation and high reproducibility
Application Data
Spike and recovery assay of human derived-extracellular vesicles from mouse EDTA plasma.
Evaluate the assay linearity using mouse EDTA plasma (2-fold diluted) spiked with Exosome, from COLO201 cells, Purified [code No. 052-09301].
1. CD9-Capture Human Exosome ELISA Kit
2. CD63-Capture Human Exosome ELISA Kit
3. CD81-Capture Human Exosome ELISA Kit
| Assay Sample | 2-fold diluted mouse EDTA plasma spiked with exosomes purified from COLO201 cells at each concentration. |
| Assay Kit | CD9/63/81-Capture Human Exosome ELISA Kit |
Human-derived exosomes were measured with good linearity from mouse EDTA plasma sample.
Comparison of extracellular vesicles expression in various human cell lines culture supernatant.
Culture supernatants of 12 types of human-derived cell lines were diluted 2, 10, 25, and 100 fold and measured with this kit.
1. CD9-Capture Human Exosome ELISA Kit
2. CD63-Capture Human Exosome ELISA Kit
3. CD81-Capture Human Exosome ELISA Kit
It was shown that the expression levels of CD9, CD63, and CD81 differed depending on the cell lines. This result was similar to the data of “Qualitative analysis of EVs purified from various cell culture supernatants.” obtained by our PS Capture Exosome ELISA Kit (Anti Mouse IgG HRP) [code No. 297-79201].
The reason of some cell lines showed no signal at 2 and 10-fold dilution is that the signal was over the detection limit.
