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CD9-Capture Human Exosome ELISA Kit (Streptavidin HRP)
In stock
Catalog ID
296-83701
Details
for Genetic Research
Anti-CD9 Antibody-immobilized 96 Well Plate 1plate, Plate Seal 4sheets, Sample Reaction Buffer 50mL, Antibody Reaction Buffer 50mL, Washing Buffer(10x) 100mL, Control Biotinylated Antibody Anti CD9(100x) 120uL, HRP-conjugated Streptavidin(100x) 240uL, TMB Solution 12uL, Stop Solution 12mL.
| Manufacturer: | FUJIFILM Wako Pure Chemical Corporation |
| GHS: |    Danger |
Kit component
| Anti-CD9 Antibody-immobilized 96 Well Plate | 8 well x 12 strips / 1 plate |
| Plate Seal | 4 sheets |
| Sample Reaction Buffer | 50 mL x 1 |
| Antibody Reaction Buffer | 50 mL x 1 |
| Washing Buffer (10x) | 100 mL x 1 |
| Control Biotinylated Antibody Anti-CD9 (100x) | 120 μL x 1 |
| HRP-conjugated Streptavidin (100x) | 240 μL x 1 |
| TMB Solution | 12 mL x 1 |
| Stop Solution | 12 mL x 1 |
Sample Preparation
Sample Dilution
Dilute samples at least twice with Sample Reaction Buffer included in the kit so that the absorbance value at 450 nm (value obtained by subtracting the absorbance at a secondary wavelength of 620 nm) is in the range of 0.2 to 2.5.
It is recommended to dilute at least 2 times for human serum and human EDTA plasma, and at least 5 times for human heparin plasma [See “Typical Data of Kit” 4.].
Consider the optimum dilution rate for the sample before performing this measurement.
Removal of microvesicles and apoptotic vesicles
Even though CD9, CD63, and CD81 have been identified as exosome markers, when performing ELISA strictly targeting exosomes, pretreatment by centrifugation should be performed to remove extracellular vesicles other than exosomes.
- Pretreatment by centrifugation
Blood Sample [serum, plasma, etc.](Before diluting with Sample Reaction Buffer)
↓ 10,000 x g, 30min., 4℃
Collect the supernatant
↓
Sample dilution step.
Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific
- For research use only. Not for use in diagnostic procedures.
- Product content may differ from the actual image due to minor specification changes etc.
- If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.
Specifications
Storage
Keep at 2-10°C
Product Inserts
Data & Technical Information
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