We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
CD9-Capture Human Exosome ELISA Kit (Streptavidin HRP) Data
Comparison of specificity to human extracellular vesicles
Specificity to human extracellular vesicle was compared between PS Capture ELISA Kit and CD9-Capture ELISA Kit.
Various concentrations of Exosomes purified from COLO201 cells (Catalog ID 052-09301) were added to two-fold diluted DMEM containing 10% FBS (ultracentrifuged), and applied to each plate of the two ELISA kits. They were detected by Anti-CD9 antibody (Clone No. 1K) that reacts with human and bovine CD9.
|
Assay sample |
2-fold diluted DMEM containing 10 % FBS (ultracentrifuged) added with each concentration of exosomes purified from COLO201 cells. |
|
Plates used |
PS Capture Plate: PS Capture Exosome ELISA Kit (Streptavidin HRP) (Catalog ID 298-80601) CD9-Capture Plate: CD9-Capture Human Exosome ELISA Kit (Streptavidin HRP) (Catyalog ID 296-83701) |
|
Detection antibody |
Anti CD9, Monoclonal Antibody(1K) (Catalog ID 014-27763). Biotin-labeled. |
PS Capture Exosome ELISA Kit (Streptavidin HRP) captured human exosomes and bovine exosomes remaining in ultracentrifuged FBS. Whereas CD9-Capture Human Exosome ELISA Kit (Streptavidin HRP) was able to capture human exosomes specifically and detect them with high sensitivity.
The detection sensitivity of COLO201 cell-derived extracellular vesicles
A standard curve was prepared using Exosome, from COLO201 cells, purified (Catalog ID 052-09301) . The detection limit and the quantification limit of this kit were calculated using the standard curve. Each standard was measured at n = 6 and Blank was measured at n = 12.
