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CD63-Capture Human Exosome ELISA Kit (Streptavidin HRP)

In stock
Catalog ID
290-83601
$990.00
Sku
290-83601
Details

for Genetic Research

Anti-CD63 Antibody-immobilized 96 Well Plate 1plate, Plate Seal 4sheets, Sample Reaction Buffer 50mL, Antibody Reaction Buffer 50mL, Washing Buffer (10x) 100mL, Control Biotinylated Antibody Anti CD63(100x) 120uL, HRP-conjugated Streptavidin(100x) 240uL, TMB Solution 12mL, Stop Solution 12mL

Manufacturer: FUJIFILM Wako Pure Chemical Corporation
GHS: Danger
Kit component
Anti-CD63 Antibody-immobilized 96 Well Plate 8 well x 12 strips / 1 plate
Plate Seal 4 sheets
Sample Reaction Buffer 50 mL x 1
Antibody Reaction Buffer 50 mL x 1
Washing Buffer (10x) 100 mL x 1
Control Biotinylated Antibody Anti-CD63 (100x) 120 μL x 1
HRP-conjugated Streptavidin (100x) 240 μL x 1
TMB Solution 12 mL x 1
Stop Solution 12 mL x 1
Sample Preparation

Sample Dilution
Dilute samples at least twice with Sample Reaction Buffer included in the kit so that the absorbance value at 450 nm (value obtained by subtracting the absorbance at a secondary wavelength of 620 nm) is in the range of 0.2 to 2.5 [See "Typical Data of Kit" 4].
Consider the optimum dilution rate for the sample before performing this measurement.

Removal of microvesicles and apoptotic vesicles
Even though CD9, CD63, and CD81 have been identified as exosome markers, when performing ELISA strictly targeting exosomes, pretreatment by centrifugation should be performed to remove extracellular vesicles other than exosomes.

  • Pretreatment procedure by centrifugation
    Blood Sample [serum, plasma, etc.) (Before dilutiong Sample Reaction Buffer)
     ↓ 10,000 x g, 30min., 4℃
    Collect the supernatant
     ↓
    To the sample dilution step.

 
Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific. 

  • For research use only. Not for use in diagnostic procedures. 
  • Product content may differ from the actual image due to minor specification changes etc. 
  • If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different. 

Specifications

Storage
Keep at 2-10°C
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