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CD63-Capture Human Exosome ELISA Kit (Streptavidin HRP)
for Genetic Research
Anti-CD63 Antibody-immobilized 96 Well Plate 1plate, Plate Seal 4sheets, Sample Reaction Buffer 50mL, Antibody Reaction Buffer 50mL, Washing Buffer (10x) 100mL, Control Biotinylated Antibody Anti CD63(100x) 120uL, HRP-conjugated Streptavidin(100x) 240uL, TMB Solution 12mL, Stop Solution 12mL
Manufacturer: | FUJIFILM Wako Pure Chemical Corporation |
GHS: | Danger |
Kit component
Anti-CD63 Antibody-immobilized 96 Well Plate | 8 well x 12 strips / 1 plate |
Plate Seal | 4 sheets |
Sample Reaction Buffer | 50 mL x 1 |
Antibody Reaction Buffer | 50 mL x 1 |
Washing Buffer (10x) | 100 mL x 1 |
Control Biotinylated Antibody Anti-CD63 (100x) | 120 μL x 1 |
HRP-conjugated Streptavidin (100x) | 240 μL x 1 |
TMB Solution | 12 mL x 1 |
Stop Solution | 12 mL x 1 |
Sample Preparation
Sample Dilution
Dilute samples at least twice with Sample Reaction Buffer included in the kit so that the absorbance value at 450 nm (value obtained by subtracting the absorbance at a secondary wavelength of 620 nm) is in the range of 0.2 to 2.5 [See "Typical Data of Kit" 4].
Consider the optimum dilution rate for the sample before performing this measurement.
Removal of microvesicles and apoptotic vesicles
Even though CD9, CD63, and CD81 have been identified as exosome markers, when performing ELISA strictly targeting exosomes, pretreatment by centrifugation should be performed to remove extracellular vesicles other than exosomes.
- Pretreatment procedure by centrifugation
Blood Sample [serum, plasma, etc.) (Before dilutiong Sample Reaction Buffer)
↓ 10,000 x g, 30min., 4℃
Collect the supernatant
↓
To the sample dilution step.
Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific.
- For research use only. Not for use in diagnostic procedures.
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