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Search results for: 'cell'
- Adoptive Cell Therapies: A Cell Culture Media Perspective
- PRIME-XV T Cell CDM
- T Cell Activation and Expansion in G-Rex Multi-Well Cell Culture Plates Protocol
- PRIME-XV T Cell Expansion XSFM
- PRIME-XV T Cell Expansion XSFM
- IS Giant Cell Tumor Conditioned Medium
- Adaptation of Adherent HEK293T Cells to Suspension Cell Culture in Chemically Defined Culture Medium
- PRIME-XV Dendritic Cell Maturation CDM Product Bulletin
- PRIME-XV T Cell CDM
- PRIME-XV NK Cell CDM
- A CHO Media Platform to Facilitate Cell Line Development
- Optimizing Cryopreservation of Hematopoietic Stem Cells Collected for Autologous Stem Cell Transplantation in Patients with Multiple Myeloma
- Development of a Serum-Free, Chemically Defined Cell Culture Medium for the Production of HIV from HUT-78 Cells
- Development of a Serum-Free, Chemically Defined Cell Culture Medium for the Production of HIV from HUT-78 Cells
- Mesenchymal Stem-Stromal Cells Poster
- Solutions For Neural Cell Cultures
- PRIME-XV NK Cell CDM
- Very Rapid Feed Medium Development for Three Different CHO Cell Lines
- High Yield Transient Protein Production in CHO Cells
- Benchtop to Bioreactor: T-Cell Culture and Expansion in Chemically Defined Media
- PRIME-XV Dendritic Cell Maturation CDM
- PRIME-XV Hematopoietic Cell Basal XSFM
- PRIME-XV Hematopoietic Cell Basal XSFM
- Developing User-Friendly Cell Culture Media
- Expanding PBMCs with PRIME-XV T Cell CDM and Shenandoah CTGrade GMP Recombinant Human Proteins in the G-Rex Cell Culture System
- Ex Vivo Expansion of Human Amniotic Fluid Stem Cells
- Mitigating Risk Through Simplified and Balanced Cell Culture Media
- Development of a Chemically Defined Growth Medium Specifically Designed for CHO DG44 Cells
- Platform Versus Specific Hydrolysate Optimization For Three CHO Cell Lines
- Characterization and Expansion of T-Cells Cultured in Xeno-Serum Free Media
- Using Protein Hydrolysates as Raw Materials for Serum-Free Cell Culture Media
- Methods of Rational Culture Media Design and Optimization for a Hybridoma and a Myeloma Cell Line
- Platform Versus Specific Hydrolysate Optimization For Three CHO Cell Lines: A Feasibility Study
- PRIME-XV Media and Cytokines for the Expansion and Differentiation of Hematopoietic Progenitor Cells
- CHO Media Platform Facilitates Integrated Cell Line Development and Media Optimization
- Cryopreservation Media Solutions for Cell and Gene Therapy Development and Manufacturing
- Q. What is the density and density gradient of ISolate , ISolate Stock Solution and ISolate Concentrate ? A. Here are the density and density gradients of ISolate. Q. Is it acceptable to put TYB in the uterus at insemination? Should TYB be washed prior to IUI? A. We recommend that the TYB freezing medium should be removed prior to IUI either by simple washing with Sperm Washing Medium or Multipurpose Handling Medium - Complete, or if washing thawed raw semen, by density gradient centr...
- Using Protein Hydrolysates as Raw Materials for Serum-Free Cell Culture Media
- Rapid Chemically-Defined Feed Media Development to Improve Simulated Cell Culture Processes
- Development of Next Generation Feed Medium to Support High Performance CHO Cell Lines
- Modulating Antibody Galactosylation through Cell Culture Medium for Improved Function and Product Quality
- Freeze, Thaw, and Expand: Scale-Up T Cell Workflow In Chemically Defined Media
- Exploring Manufacturing Platforms and Ancillary Materials to Address Cell and Gene Therapy Product Needs
- A Versatile and Scalable Media Platform Designed for Cell Based Applications Using Human Embryo
- A Chemically-Defined, Animal Component-Free Ex Vivo Expansion Process for Activated Human T Cells
- Development of DMSO-Free Chemically Defined Cryopreservation Solution for Human-Derived Mesenchymal Stem/Stromal Cells
- Cryopreservation Protocol for PBMCs and T Cells Using PRIME-XV FreezIS DMSO-Free Cryopreservation Medium
- Development of Serum-Free Media for Mesenchymal Stem Cells, Complied with Japanese Standards for Biological Ingredients
- Case Study: Comparison of Cost and Efficacy of Complete Cell Culture Media vs. Home-Brew Media
- Lipid Mediated Th1 Skewing of Human Dendritic Cells Cultured in a Chemically-Defined, Animal Component-Free Medium
- Very Rapid Development Of A Chemically-Defined Feed Medium For A Specific Cell Line Using Spent Media Analysis
- Q. Why was L-Glutamine eliminated from many of your formulas? A. L-Glutamine is easily hydrolyzed in liquid and byproducts of hydrolysis can be toxic to the cell. Additionally, the necessary concentration of L-Glutamine may be compromised after a period of time. Since L-Glutamine is an essential amino acid, it is best for the end users to supplement media with the recommended concentration of fresh L-Glutamine . Q. What effects could freezing and thawing have on my sera? A. We generally di...
- Q. How do we prevent mycoplasma contamination in cell culture? A. Good aseptic techniques should be used in all laboratory applications. The best method for prevention is to test all materials that come in contact with the cells. This should be done on a regular basis to ensure that mycoplasma is not introduced into the cell culture and to identify any potential sources of contamination. Q. Should I be concerned with mycoplasma in my cell culture? A. Mycoplasma contamination should always b...
- Q. What are the differences between the CHANG Medium formulations? A. CHANG Medium C, D, In Situ and Amnio are formulated specifically for prenatal cytogenetic testing of amniocytes, CVS (Chorionic Villus Sampling) and POCs (Products of Conception). Q. What is the proper way to reconstitute the CHANG Medium C lyophilized supplement? A. First allow the supplement to equilibrate to room temperature. Once equilibrated, under aseptic conditions the appropriate amount of sterile, distill...
- Q. How does FUJIFILM Irvine Scientific define serum-free, animal-component free, protein-free, and chemically-defined media? A. Here is how FUJIFILM Irvine Scientific defines these types of media: Serum-free media do not contain or require the addition of serum. Protein-free media do not contain any proteins but can contain undefined peptides from plant hydrolysates. FUJIFILM Irvine Scientific animal-component free products are manufactured with raw materials that do not contain any anim...
- Q. Is additional L-glutamine or other supplementation required? A. IS Sf Insect is a complete, ready-to-use medium. Additional supplementation is not required. However, it can be further supplemented according to user needs. Q. What is the L-glutamine concentration in IS Sf Insect? A. The medium contains 8 mM L-Glutamine. Q. What is the glucose level in IS Sf Insect? A. IS Sf Insect contains 8 gm/L glucose. Q. Does the medium contain a sodium bicarbonate or other buffering s...
- Q. What is the glucose concentration in the HEK 293 medium and feed? A. BalanCD HEK 293 medium contains 6 g/L glucose and BalanCD HEK293 Feed contains 40 g/L glucose. Q. How should adherent HEK 293 cells (banked in a FCS containing medium) be adapted into suspension in BalanCD HEK 293 medium? A. We recommend starting with adapting adherent HEK cells to suspension culture in BalanCD HEK 293 medium with serum (the same concentration the cells have been adapted to). Follow the standard pro...
- Q. Is it possible to add protein, such as Serum Substitute Supplement (SSS) or Human Serum Albumin to complete media? A. Our complete media contain the concentration of protein indicated below; it is possible to add more protein if needed, as long as you consider that the following has already been added. Multipurpose Handling Media-Complete (MHM-C) with Gentamicin is pre-supplemented with HSA for a final protein concentration of 5 mg/mL. Continuous Single Culture-NX Complete (CSCM-N...
- Q. Why would I heat-inactivate my serum? A. Heat inactivation is a process used to inactivate protein complement components that are present in the serum. The proteins from the complement cascade have the potential to impact or interfere with certain cellular processes.