Search results for: 'cell culture medium'

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Modulating Antibody Galactosylation through Cell Culture Medium for Improved Function and Product Quality
10740icc_rev0_poster_from_esact_2017_on_modulating_antibody_galactosylation.pdf
Development of a Serum-Free, Chemically Defined Cell Culture Medium for the Production of HIV from HUT-78 Cells
irc_is_vaccine_tech_poster_606.pdf
Development of a Serum-Free, Chemically Defined Cell Culture Medium for the Production of HIV from HUT-78 Cells
irc_is_vaccine_tech_poster_606_1.pdf
Gamete, Fertilization, and Embryo Culture Media FAQ
Q. Is it possible to add protein, such as Serum Substitute Supplement (SSS) or Human Serum Albumin to complete media? A. Our complete media contain the concentration of protein indicated below; it is possible to add more protein if needed, as long as you consider that the following has already been added. Multipurpose Handling Media-Complete (MHM-C) with Gentamicin is pre-supplemented with HSA for a final protein concentration of 5 mg/mL. Continuous Single Culture-NX Complete (CSCM-N...
Lipid Mediated Th1 Skewing of Human Dendritic Cells Cultured in a Chemically-Defined, Animal Component-Free Medium
005459_lipid_mediated_th1_skewing_of_human_dendritic_cells_scientific_poster.pdf
OPEN ACCESS: Qu, C. et al. (2020) Evaluation of growth, stemness, and angiogenic properties of dental pulp stem cells cultured in cGMP xeno-/serum-free medium. Cell and Tissue Research.
OPEN ACCESS: Muraya, Koji et al. (2019) Enhancement of cellular adhesion and proliferation in human mesenchymal stromal cells by the direct addition of recombinant collagen I peptide to the culture medium. BioResearch.
Adaptation of Adherent HEK293T Cells to Suspension Cell Culture in Chemically Defined Culture Medium
014223-adaptation-of-adherent-hek293t-cells-to-suspension.pdf
Sperm Processing Media FAQ
Q. What is the density and density gradient of ISolate , ISolate Stock Solution and ISolate Concentrate ? A. Here are the density and density gradients of ISolate. Q. Is it acceptable to put TYB in the uterus at insemination? Should TYB be washed prior to IUI? A. We recommend that the TYB freezing medium should be removed prior to IUI either by simple washing with Sperm Washing Medium or Multipurpose Handling Medium - Complete, or if washing thawed raw semen, by density gradient centr...
CHANG Media FAQ
Q. What are the differences between the CHANG Medium formulations? A. CHANG Medium C, D, In Situ and Amnio are formulated specifically for prenatal cytogenetic testing of amniocytes, CVS (Chorionic Villus Sampling) and POCs (Products of Conception). Q. What is the proper way to reconstitute the CHANG Medium C lyophilized supplement? A. First allow the supplement to equilibrate to room temperature. Once equilibrated, under aseptic conditions the appropriate amount of sterile, distill...
Bioproduction Serum-Free Products FAQ
Q. Why was L-Glutamine eliminated from many of your formulas? A. L-Glutamine is easily hydrolyzed in liquid and byproducts of hydrolysis can be toxic to the cell. Additionally, the necessary concentration of L-Glutamine may be compromised after a period of time. Since L-Glutamine is an essential amino acid, it is best for the end users to supplement media with the recommended concentration of fresh L-Glutamine . Q. What effects could freezing and thawing have on my sera? A. We generally di...
Insect Media FAQ
Q. Is additional L-glutamine or other supplementation required? A. IS Sf Insect is a complete, ready-to-use medium. Additional supplementation is not required. However, it can be further supplemented according to user needs. Q. What is the L-glutamine concentration in IS Sf Insect? A. The medium contains 8 mM L-Glutamine. Q. What is the glucose level in IS Sf Insect? A. IS Sf Insect contains 8 gm/L glucose. Q. Does the medium contain a sodium bicarbonate or other buffering s...
HEK293 System FAQ
Q. What is the glucose concentration in the HEK 293 medium and feed? A. BalanCD HEK 293 medium contains 6 g/L glucose and BalanCD HEK293 Feed contains 40 g/L glucose. Q. How should adherent HEK 293 cells (banked in a FCS containing medium) be adapted into suspension in BalanCD HEK 293 medium? A. We recommend starting with adapting adherent HEK cells to suspension culture in BalanCD HEK 293 medium with serum (the same concentration the cells have been adapted to). Follow the standard pro...
General Bioproduction Products FAQ
Q. How does FUJIFILM Irvine Scientific define serum-free, animal-component free, protein-free, and chemically-defined media? A. Here is how FUJIFILM Irvine Scientific defines these types of media: Serum-free media do not contain or require the addition of serum. Protein-free media do not contain any proteins but can contain undefined peptides from plant hydrolysates. FUJIFILM Irvine Scientific animal-component free products are manufactured with raw materials that do not contain any anim...