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Search results for: 'protocols'
- Sperm Separation Protocols
- Sperm Thawing Protocols
- Comparison of two sperm freezing protocols to preserve human sperm quality
- Simplified Oocyte Vitrification Protocol
- HSV Device Loading Protocol
- Impulse Heat Sealer Validation Protocol
- Simplified Warming Protocol
- Culture Of Embryos Protocol: Post - Vitrification and Warming
- CHANG Marrow Recommended Protocol
- Protein Concentration Protocol (MHM)
- Simplified Embryo Vitrification Protocol
- CHANG Medium MF Recommended Protocol
- Semen and Sperm Freezing Protocol
- Collapsing Protocol for Human Blastocysts
- Single-Step Uninterrupted Culture Medium Protocol
- CHANG Amnio Recommended Protocol for CVS
- CHANG Amnio Recommended Protocol for AFC
- Uninterrupted Culture Media Complete (CSCM-NXC and CSCM-C) Recommended Protocol Microdrop
- Oocyte Vitrification Protocol with Vit Kit - Freeze NX
- Embryo Vitrification Protocol with Vit Kit - Freeze NX
- Uninterrupted Culture Media Complete (CSCM-NXC and CSCM-C) Recommended Protocol 4/5 Well Dish
- Warming Oocytes and Embryos Protocol with Vit Kit - Warm NX
- Uninterrupted Culture Media (CSCM-NX and CSCM) Recommended Protocol Microdrop
- Universal Warming Protocol For a Transnational Egg Donation Program with Vitrified Oocytes
- Uninterrupted Culture Media (CSCM-NX and CSCM) Recommended Protocol 4/5-Well Dish
- Cryopreservation Protocol for PBMCs and T Cells Using PRIME-XV FreezIS DMSO-Free Cryopreservation Medium
- T Cell Activation and Expansion in G-Rex Multi-Well Cell Culture Plates Protocol
- A Low Lactate Undisturbed Culture Medium Protocol Provides an Increase in Usable Blastocysts on Day 5 vs. Day 6
- Q. What is the density and density gradient of ISolate , ISolate Stock Solution and ISolate Concentrate ? A. Here are the density and density gradients of ISolate. Q. Is it acceptable to put TYB in the uterus at insemination? Should TYB be washed prior to IUI? A. We recommend that the TYB freezing medium should be removed prior to IUI either by simple washing with Sperm Washing Medium or Multipurpose Handling Medium - Complete, or if washing thawed raw semen, by density gradient centr...
- Q. Why was L-Glutamine eliminated from many of your formulas? A. L-Glutamine is easily hydrolyzed in liquid and byproducts of hydrolysis can be toxic to the cell. Additionally, the necessary concentration of L-Glutamine may be compromised after a period of time. Since L-Glutamine is an essential amino acid, it is best for the end users to supplement media with the recommended concentration of fresh L-Glutamine . Q. What effects could freezing and thawing have on my sera? A. We generally di...
- Q. How long after opening bottles can we use the products? A. When stored as directed, products are stable until the expiration date shown on the bottle. Upon opening the bottles, refer to the product insert for the recommended usage time. Q. Is it ok to aliquot into smaller vials? A. As long as the clinic works in an aseptic, sterile environment, the products can be aliquoted into smaller bottles or vials and stored per the directions. Please note that we cannot guarantee that the aliquots...
- Q. Is it possible to add protein, such as Serum Substitute Supplement (SSS) or Human Serum Albumin to complete media? A. Our complete media contain the concentration of protein indicated below; it is possible to add more protein if needed, as long as you consider that the following has already been added. Multipurpose Handling Media-Complete (MHM-C) with Gentamicin is pre-supplemented with HSA for a final protein concentration of 5 mg/mL. Continuous Single Culture-NX Complete (CSCM-N...
- Q. Is additional L-glutamine or other supplementation required? A. IS Sf Insect is a complete, ready-to-use medium. Additional supplementation is not required. However, it can be further supplemented according to user needs. Q. What is the L-glutamine concentration in IS Sf Insect? A. The medium contains 8 mM L-Glutamine. Q. What is the glucose level in IS Sf Insect? A. IS Sf Insect contains 8 gm/L glucose. Q. Does the medium contain a sodium bicarbonate or other buffering s...
- Q. What is the glucose concentration in the HEK 293 medium and feed? A. BalanCD HEK 293 medium contains 6 g/L glucose and BalanCD HEK293 Feed contains 40 g/L glucose. Q. How should adherent HEK 293 cells (banked in a FCS containing medium) be adapted into suspension in BalanCD HEK 293 medium? A. We recommend starting with adapting adherent HEK cells to suspension culture in BalanCD HEK 293 medium with serum (the same concentration the cells have been adapted to). Follow the standard pro...
- Q. How does FUJIFILM Irvine Scientific define serum-free, animal-component free, protein-free, and chemically-defined media? A. Here is how FUJIFILM Irvine Scientific defines these types of media: Serum-free media do not contain or require the addition of serum. Protein-free media do not contain any proteins but can contain undefined peptides from plant hydrolysates. FUJIFILM Irvine Scientific animal-component free products are manufactured with raw materials that do not contain any anim...