Anti Iba1, Rabbit (for Immunocytochemistry)

Iba1 (Ionized calcium-binding adapter molecule1) is an approximately 17 kDa calcium-binding protein. It is used as a microglial marker because it is expressed specifically in microglia in the central nervous system¹). It is expressed in both resting and activated microglia, but is reportedly expressed more highly in activated microglia²). It is also expressed in macrophages in peripheral tissues and is known as AIF-1 (Allograft inflammatory factor-1). Iba1 binds to F-actin in cells to form actin bundles. The formation of actin bundles is thought to be required for the membrane ruffling observed during cell migration and phagocytosis³).

Fujifilm Wako’s "Anti Iba1, Rabbit (for immunocytochemistry)" (Product Number 019-19741), which allows even microglia processes to be stained by immunohistochemical staining, is used by researchers all over the world as a microglia marker antibody standard.

• Enables even the processes of microglia to be immunohistochemical-stained*.
• Relatively small amount needed, with concentrations ranging from 1:500 to 1:1,000.

* The result of staining depends on sample condition. Staining performance is not guaranteed.

for Immunochemistry

**Experience with humans⁴ dogs⁵, cats⁶, pigs⁷, marmosets⁸, zebrafish⁹, etc. has been reported.

Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific.

• For research use only. Not for use in diagnostic procedures.
• Product content may differ from the actual image due to minor specification changes etc.
• If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

1. Imai, Y., Ibata, I., Ito, D., Ohsawa, K. & Kohsaka, S.: Biochemical and biophysical research communications, 224(3), 855(1996).
   A Novel Geneiba1 in the Major Histocompatibility Complex Class III Region Encoding an EF Hand Protein Expressed in a Monocytic Lineage
2. Mori, I., Imai, Y., Kohsaka, S. & Kimura, Y.: Microbiology and immunology, 44(8), 729(2000).
   Upregulated expression of Iba1 molecules in the central nervous system of mice in response to neurovirulent influenza A virus infection
3. Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. & Imai, Y.: Biochemical and biophysical research communications, 286(2), 292(2001).
   Iba1 is an actin-cross-linking protein in macrophages/microglia.
4. Zhao, S. et al.: Cell, 180(4), 796(2020).
   Cellular and Molecular Probing of Intact Human Organs
5. Ahn, J.H. et al.: Lab. Anim. Res., 28(3), 165 (2012).
   Comparison of alpha-synuclein immunoreactivity in the spinal cord between the adult and aged beagle dog
6. Ide, T. et al.: J. Vet. Med .Sci., 72(1), 99 (2010).
   Histiocytic Sarcoma in the Brain of a Cat
7. Gaige, S. et al.: Neurotoxicology, 34, 135(2013).
   c-Fos immunoreactivity in the pig brain following deoxynivalenol intoxication: Focus on NUCB2/nesfatin-1 expressing neurons
8. Rodriguez-Callejas, J.D. et al.: Front. Aging Neurosci., 8, 315(2016).
   Evidence of Tau Hyperphosphorylation and Dystrophic Microglia in the Common Marmoset
9. Fantin, A. et al.: Blood, 116(5), 829(2010).
   Tissue macrophages act as cellular chaperones for vascular anastomosis downstream of VEGF-mediated endothelial tip cell induction