Immunohistochemistry of frozen section in mouse model of Alzheimer’s disease (APP^NL-G-F mouse)　brain cerebral neocortex using anti Iba1, goat polyclonal antibody at dilution of 1:1,000, and anti goat IgG, Alexa Flour 488-conjugated. Aβ were stained by 0.001% FSB solution(Amyloid fluorescent probe). The data were provided by Dr. Sakakibara, National Center for Geriatrics and Gerontology in Japan.

Immunohistochemistry of wild type mouse brain hippocampus using anti Iba1, goat polyclonal antibody(1:200), and anti goat IgG, Alexa Flour 488-conjugated. Nuclear were stained by Hoechst33342.
The data were provided by Dr. Takata, Kyoto Pharmaceutical University in Japan.

Immunohistochemistry of frozen section in rat (left) and mouse (right) brain cerebral cortex using anti Iba1, goat polyclonal antibody at dilution of 1:250, and anti goat IgG, Alexa Flour 488-conjugated.
The data were provided by Dr. Nakajima from Soka University in Japan.

Sample: paraffin section of mouse brain frontal lobe
1st antibody: Anti Iba1, goat（1:1.000）
2nd antibody: Anti goat IgG, biotin-conjugated
Antigen retrieval: 10mM citric acid buffer(pH6.0), 90℃, 10min

The data were provided by Dr. Nakajima from Soka University in Japan.

Sample:
Microglia from rat primary culture 10μg
Neuron from rat primary culture 10μg
Astrocyte from rat primary culture 10μg
Cerebral cortex from rat brain 100μg

1st antibody: Anti Iba1, goat（1:1.000）
2nd antibody: Anti goat IgG, HRP-conjugated