Anti Human Ago2, Monoclonal Antibody

This product is for research use only. Do not administer it to human.
Argonaute2 (AGO2) was isolated as one of the main components of RISC (RNA-induced silencing complex). AGO2 captures siRNA and microRNA which are working as a guide molecule for interaction with target mRNAs in RNAi pathway. In this pathway, AGO2 catalyzes the nicking of target mRNAs and binding between RISC and target mRNAs.

This monoclonal antibody is not only used for western blot and immunohistochemistry, but also immunoprecipitation of hAGO2.

Monoclonal Anti human AGO2 antibody is produced by hybridoma clone 4G8, established by fusing mouse myeloma cells P3U1 and lymphocytes of BALB/c mice immunized with recombinant human AGO2 produced in E. coli This antibody was purified by an affinity column from cell culture supernatant.

[Concentration] Indicated on each label.
[Formulation] 1 x TBS (pH 7.4) aqueous solution with 10% glycerol containing 0.09% sodium azide
[Clone No.] 4G8
[Specificity] Specific to human AGO2 [Subclass] IgG1
[Working Dilution]

Western blot 1:100 ~ 1:200

Immunoprecipitation 10 μg/20 μL 10% Protein G beads slurry

Immunocytochemistry 1:20 ~ 1:50

[Note] Avoid repeated freezing and thawing.
[Product List for microRNA Research]

Features

1) For IP, Western Blot
2) Cross reactivity with AGO2 protein of rat and hamster
3) For purification of RNA captured by RISC

for Immunochemistry

microRNA Purification kits based on Immunoprecipitation method using antibody against Argonaute protein

Preparation kits of cDNA encoding microRNA

Antibodies

Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific.

• For research use only. Not for use in diagnostic procedures.
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