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Anti CD9, Rat Monoclonal Antibody (77B) Application Data
ELISA
Immobilized this product and competitors anti CD9 antibodies, and then compared the capture performance using ELISA.
| Immobilized antibodies | Anti CD9, Rat Monoclonal Antibody (77B), Competitor’s anti CD9 antibody (each 5 μg/mL) |
| Exosome samples | Exosome, from COLO201 cells, purified (Catalog ID: 052-09301) |
| Detection antibody | Anti CD9, Rat Monoclonal Antibody (77B), Biotin-conjugated |
This product shows higher signal value and S/B ratio than competitors.
Western Blot
CD9 proteins from exosomes were detected by western blot.
| Sample | Exosomes from cell culture supernatant of COLO201, human serum and human heparin plasma |
| Primary antibody | Anti CD9, Rat Monoclonal Antibody (77B) (1 μg/mL) |
| Secondary antibody | Anti Rat IgG(H+L), Donkey, Peroxidase-conjugated [Code No. 564-73961] (×20,000 diution) |
| Detection reagent | Immunostar Zeta [Code No. 291-72401] |
CD9 protein (24kDa) in each sample (non-reducing condition) was detected with this product.
