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Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated Application Data
ELISA
Directly Detection of exosomes in biofluids
Exosomes in biofluids were detected using PS Capture Exosome ELISA Kit (Streptavidin HRP) (Catalog ID: 298-80601). Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) was used as a primary antibody. Then, HRP-conjugated Streptavidin and TMB solution in the ELSIA kit were used for detection.
Sample | Human serum or human heparin plasma |
Primary antibody | Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) (1:4,000) |
Detection | HRP-conjugated streptavidin and TMB solution in the ELISA kit |
Exosomes in human serum and human heparin plasma were detected concentration-dependently with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953).
Directly Detection of exosomes in cell culture supernatant
Exosomes in cell culture supernatant of COLO201 cells were detected using PS Capture Exosome ELISA Kit (Streptavidin HRP) (Catalog ID: 298-80601). As primary antibodies, Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) and three competitive anti CD9 antibodies (biotin conjugated) were used. Then, HRP-conjugated Streptavidin and TMB solution in the ELSIA kit were used for detection.
Sample | Cell culture supernatant of COLO201 |
Primary antibody | Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) (1:4,000) and competitive biotin conjugated antibodies |
Detection | HRP-conjugated streptavidin and TMB solution in the ELISA kit |
Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953).showed the best signal/background ratio.
Western Blot
Detection of exosomes isolated from serum, heprin plasma and COLO201 cells
CD9 proteins on exosomes isolated using MagCapture Exosome Isolation Kit PS (Product Code: 293-77601) were detected with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) using a Western Blot method in non-reducing conditions.
Sample | Exosomes from human serum, human heparin plasma and cell culture supernatant of COLO201 |
Gel | SuperSep Ace, 10-20%, 17well (Product code: 198-15041) |
Primary antibody | Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) (1:1,000) |
Detection | HRP-Streptavidin and ImmunoStar Zeta (Product Code: 295-72404) |
CD9 protein (24kDa) in each samples was detected with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953).