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Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated
CD81 is 26 kDa cell surface protein and is known as TAPA-1 (Target of the Antiproliferative Antibody 1) and Tetraspanin-28 (Tspan-28). CD81 interacts directly with immunoglobulin superfamily member 8 (IGSF8, CD316) and CD36. It also forms a signal transduction complex with CD19, CD21 and Leu-13 (CD225) on the surface of B cell. In addition, on T cells CD81 associates with CD4 and CD8 and provides a costimulatory signal with CD3.
Anti CD81, Rat Monoclonal Antibody (9B) is established against human CD81 with Rat.
This antibody is labeled with biotin.
For Immunochemistry
Manufacturer: | FUJIFILM Wako Pure Chemical Corporation |
Cross Reactivity: | Human Note: This antibody doesn’t recognize the mouse and rat CD81, but slightly recognize bovine CD81. |
Clone No.: | 9B |
Host: | Rat |
Subclass: | IgG2b |
Formulation: | 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.2 |
Label: | Biotin |
Evaluating a reactivity:
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".
Application
ELISA: | 1: 2,000 – 1: 16,000 |
Western Blot: | 1: 1,000 - 1: 8,000 *Please use this antibody under non-reducing condition |
Immunoprecipitation: | 5-20 μg/assay |
Please optimize the most appropriate concentration for your analysis and samples.
Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific.
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