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Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated Application Data
Detection of exosomes by ELISA
Detection sensitivity of EVs was compared using ELISA kit of our product.
| EVs Sample | Exosomes, from COLO201 cells, purified (Catalog ID 052-09301) |
| ELISA Kit | PS Capture Exosome ELISA Kit (Streptavidin HRP)(Catalog ID 298-80601) |
| Primary Antibody | Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated (Catalog ID 011-28111) and Competitive biotin-conjugated antibodies (each 250 ng/mL) |
This product is lower blank value and higher EVs signal value than competitor antibodies.
Directly detection of exosomes in biofluids by ELISA
Using this product as primary antibody, Exosomes from biofluids samples was detected by ELISA kit of our product.
| Sample | Human serum and EDTA plasma |
| ELISA Kit | PS Capture Exosome ELISA Kit (Streptavidin HRP)(Catalog ID 298-80601) |
| Primary Antibody | Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated (Catalog ID 011-28111) (250 ng/mL) |
Exosomes in biofluids were detected concentration-dependently with this product.
Western Blot
CD81 proteins on exosomes isolated using our kit were detected with this product using western blot method in non-reducing conditions.
| Sample | Exosomes from cell culture supernatant of COLO201, human serum and human heparin plasma |
| Exosome Isolation | MagCapture Exosome Isolation Kit PS (Catalog ID 293-77601) |
| Detection Antibody | Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated (Catalog ID 011-28111) (250 ng/mL) |
| Detection Reagent | HRP-Streptavidin, ImmunoStar Zeta (Catalog ID 291-72401) |
CD81 protein (26kDa) in each samples was detected with this product.
