Exosomes (1.25 to 20 ng) isolated and purified from COLO201 cell conditioned medium using MagCapture Exosome Isolation Kit PS (Code No. 293-77601) were detected with this antibody or competitor A.

Isolation of Exosomes: MagCapture Exosome Isolation Kit PS (Code No. 293-77601)
Gel: SuperSep ACE 10-20%, 17well (Code No. 198-15041)
Runnning Buffer: SDS-PAGE Buffer, pH8.5 (Code No. 192-16801)
Blocking: 3% Skim milk / PBS-T
Chemiluminescent reagent: ImmunoStar Zeta (Code No. 295-72404)
Primary Antibody: 2,000-fold dilution
Secondary Antibody: Anti Mouse IgG (H+L), Peroxidase Conjugated, 10,000-fold dilution.

Exosomes in pretreated* 500-fold diluted COLO201 cell conditioned medium were measured using this antibody or competitor A as the primary antibody. As reagents other than the primary antibody, ones included in PS Capture Exosome ELISA Kit (Anti Mouse IgG POD) (Code No. 297-79201) were used.

*Pretreatment condition: 10,000 x g, 30 min.

From pretreated* 8-fold diluted COLO201 cell conditioned medium, exosomes were isolated using PS Capture Exosome Flow Cytometry Kit (Catalog ID 297-79701) and subjected to detection of the exosome surface antigen by flow cytometry using this antibody or competitor A and fluorescence-labeled anti-mouse IgG antibody (Code No. 569-79631).

*Pretreatment condition: 10,000 x g, 30 min.

Exosomes in pretreated* COLO201 cell conditioned medium were captured by immunoprecipitation using this antibody or competitor A and detected using HRP-labeled anti-CD9 antibody (competitor B).

*Pretreatment condition: 10,000 x g, 3 min.

Gel: SuperSep ACE 10-20%, 17well (Code No. 198-15041)
Running Buffer: SDS-PAGE Buffer, pH8.5 (Code No. 192-16801)
Blocking: 3% Skim milk/PBS-T
Chemiluminescent reagent: ImmunoStar Zeta (Code No. 295-72404)
HRP conjugated Anti-CD81 antibody: 1,000-fold dilution
COLO201 cell culture supernatant: 1 mL
Antibody amount: 5 μg

This product successfully detected CD81 on the exosome surface at a high sensitivity. Also, CD81 positive exosomes were efficiently collected.