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Anti CD63, Monoclonal Antibody (3-13) Application Data
Exosomes were purified from COLO201 cell culture supernatant and used for comparison of detection sensitivity of Western Blotting.
This product was capable of detecting CD63 protein on exosome surface at a sensitivity higher than those of Competitors A and B.
| Exosome isolation | MagCapture Exosome Isolation Kit PS (Code No. 293-77601) |
| Gel | SuperSep Ace 5-20%, 17 wells (Code No. 194-15021) |
| Running buffer | SDS-PAGE Buffer, pH8.5 (Code No. 192-16801) |
| Blocking | 3% Skimmed milk/PBS-T |
| Chemiluminescent reagent | ImmunoStar Zeta (Code No. 295-72404) |
| Primary antibody | diluted 1,000-fold |
| Secondary antibody | anti-mouse IgG (H+L, peroxidase-conjugated, diluted 10,000- fold) (for Competitor B, the secondary antibody included in the product was used) |
