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Control Standard Endotoxin
Our Control Standard Endotoxin (CSE) is a lyophilized product composed of a 500-ng vial of endotoxin, purified from E. coli UKT-B strain that serves as an accurate and dependable standard for endotoxin testing.
Key Features
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Endotoxin derived from E. coli UKT-B
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Can be used to prepare controls and standard curves
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Potency of CSE supplied to match each lot of LAL
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Reconstituted CSE can be stored at 2–10°C for 1 month
Catalog Number |
Contents |
CSE4037-5006 |
6 vials (500 ng/vial) |
Control Standard Endotoxin
Q: How should the CSE be prepared?
A: The reconstitution volume of the CSE depends on the reagent lot it will be paired with in testing. Whether the product is PYROSTAR ES-F Series or a Limulus Color KY series, a COA associated with the reagent lot will list the information for the associated CSE lot. The COA is provided or can be accessed at www.wakopyrostar.com for each reagent lot.
Note: When testing with the Limulus Color KY series, the potency is shown on the CSE vial label. Divide the potency (EU/vial) by 1,000 to calculate the volume of LRW needed to reconstitute the CSE for a yield 1,000 EU/mL.
Q: How should the dilution for the gel clot assay be prepared?
A: The dilution series for gel clot assay are two-fold dilutions of the CSE. Based on the reagent’s sensitivity (λ), the two-fold dilutions generate a standard curve of 2 λ, λ, λ/2 and λ/4.
Example: PYROSTAR ES-F multi-test reagent labeled sensitivity (λ): 0.015 EU/mL
The final endotoxin (dilution tubes 8, 9, 10 and 11) would be achieved to generate a standard of dilutions to test for the labeled sensitivity of 0.015 EU/mL.
Note: This is an example of how to perform the two-fold dilution. Adjusting the volumes of the LRW and endotoxin is acceptable if the ratios are maintained.
Initial Endotoxin (EU/mL) |
Volume of LRW added (mL) | Volume of Endotoxin added (mL) |
Final Endotoxin (EU/mL) |
1000 |
3.6 |
0.4 |
100 |
100 |
3.6 |
0.4 |
10 |
10 |
3.6 |
0.4 |
1 |
1 |
2.0 |
2.0 |
0.5 |
0.5 |
2.0 |
2.0 |
0.25 |
0.25 |
2.0 |
2.0 |
0.125 |
0.125 |
2.0 |
2.0 |
0.06 |
0.06 |
2.0 |
2.0 |
0.03 |
0.03 |
2.0 |
2.0 |
0.015 |
0.015 |
2.0 |
2.0 |
0.0078 |
0.0078 |
2.0 |
2.0 |
0.0039 |
Q: How should dilutions for the kinetic assays be prepared?
A: The dilution series for kinetic assays are typically ten-fold dilutions of the CSE. Based on the reagent’s range the tenfold dilutions generate a standard curve with at least three standards.
Example: PYROSTAR ES-F multi-test reagent labeled sensitivity (λ): 0.015 EU/mL has a KTA range of 0.001-10 EU/mL. To reach the highest possible sensitivity, the dilution would be shown as below. The final endotoxin (dilution tubes 4, 5 and 6) would be used to generate the standard curve for KTA testing.
Note: This is an example of how to perform a ten-fold dilution. Adjusting the volumes of the LRW and endotoxin is acceptable as long as the ratios are maintained.
Initial Endotoxin (EU/mL) |
Volume of LRW added (mL) | Volume of Endotoxin added (mL) | Final Endotoxin (EU/mL) |
1000 (CSE vials are reconstitution) |
3.6 | 0.4 |
100 (dilution tube 1) |
100 | 3.6 | 0.4 |
10 (dilution tube 2) |
10 | 3.6 | 0.4 |
1 (dilution tube 3) |
1 | 3.6 | 0.4 |
0.1 (dilution tube 4) |
0.1 | 3.6 | 0.4 |
0.01 (dilution tube 5) |
0.01 | 3.6 | 0.4 |
0.001 (dilution tube 6) |