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Control Standard Endotoxin

In stock
Catalog ID
CSE4037-5006
Stock In Stock
$56.19
Sku
CSE4037-5006
Details

Our Control Standard Endotoxin (CSE) is a lyophilized product composed of a 500-ng vial of endotoxin, purified from E. coli UKT-B strain that serves as an accurate and dependable standard for endotoxin testing.

Key Features

  • Endotoxin derived from E. coli UKT-B

  • Can be used to prepare controls and standard curves

  • Potency of CSE supplied to match each lot of LAL

  • Reconstituted CSE can be stored at 2–10°C for 1 month

Catalog Number

Contents

CSE4037-5006

6 vials (500 ng/vial)

Control Standard Endotoxin

Q: How should the CSE be prepared?

A: The reconstitution volume of the CSE depends on the reagent lot it will be paired with in testing. Whether the product is PYROSTAR ES-F Series or a Limulus Color KY series, a COA associated with the reagent lot will list the information for the associated CSE lot. The COA is provided or can be accessed at www.wakopyrostar.com for each reagent lot.

Note: When testing with the Limulus Color KY series, the potency is shown on the CSE vial label. Divide the potency (EU/vial) by 1,000 to calculate the volume of LRW needed to reconstitute the CSE for a yield 1,000 EU/mL.

Q: How should the dilution for the gel clot assay be prepared?

A: The dilution series for gel clot assay are two-fold dilutions of the CSE. Based on the reagent’s sensitivity (λ), the two-fold dilutions generate a standard curve of 2 λ, λ, λ/2 and λ/4.

Example: PYROSTAR ES-F multi-test reagent labeled sensitivity (λ): 0.015 EU/mL

The final endotoxin (dilution tubes 8, 9, 10 and 11) would be achieved to generate a standard of dilutions to test for the labeled sensitivity of 0.015 EU/mL.

Note: This is an example of how to perform the two-fold dilution. Adjusting the volumes of the LRW and endotoxin is acceptable if the ratios are maintained.

 

Initial Endotoxin (EU/mL)

Volume of LRW added (mL) Volume of Endotoxin added (mL)

Final Endotoxin (EU/mL)

1000
(CSE vials after reconstitution)

3.6

0.4

100
(dilution tube 1)

100

3.6

0.4

10
(dilution tube 2)

10

3.6

0.4

1
(dilution tube 3)

1

2.0

2.0

0.5
(dilution tube 4)

0.5

2.0

2.0

0.25
(dilution tube 5)

0.25

2.0

2.0

0.125
(dilution tube 6)

0.125

2.0

2.0

0.06
(dilution tube 7)

0.06

2.0

2.0

0.03
(dilution tube 8)

0.03

2.0

2.0

0.015
(dilution tube 9)

0.015

2.0

2.0

0.0078
(dilution tube 10)

0.0078

2.0

2.0

0.0039
(dilution tube 11)

 

Q: How should dilutions for the kinetic assays be prepared?

A: The dilution series for kinetic assays are typically ten-fold dilutions of the CSE. Based on the reagent’s range the tenfold dilutions generate a standard curve with at least three standards.

Example: PYROSTAR ES-F multi-test reagent labeled sensitivity (λ): 0.015 EU/mL has a KTA range of 0.001-10 EU/mL. To reach the highest possible sensitivity, the dilution would be shown as below. The final endotoxin (dilution tubes 4, 5 and 6) would be used to generate the standard curve for KTA testing.

Note: This is an example of how to perform a ten-fold dilution. Adjusting the volumes of the LRW and endotoxin is acceptable as long as the ratios are maintained.

 

Initial Endotoxin (EU/mL)

Volume of LRW added (mL) Volume of Endotoxin added (mL) Final Endotoxin (EU/mL)

1000

(CSE vials are reconstitution)

3.6 0.4

100

(dilution tube 1)

100 3.6 0.4

10

(dilution tube 2)

10 3.6 0.4

1

(dilution tube 3)

1 3.6 0.4

0.1

(dilution tube 4)

0.1 3.6 0.4

0.01

(dilution tube 5)

0.01 3.6 0.4

0.001

(dilution tube 6)

Specifications

Storage
Reconstituted CSE can be stored at 2-10°C for 1 month.
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