- What is the difference between the CHANG MEDIUM® formulations?
- How long can I store CHANG MEDIUM® once the supplement is added to the basal medium?
- What is the proper way to reconstitute the CHANG MEDIUM® C lyophilized supplement?
- What quality control is performed on CHANG MEDIUM® formulations?
- What is "AmnioStat-FLM®-PG"?
- How is the AmnioStat-FLM®-PG kit configured?
- My AmnioStat-FLM®-PG kit was left at room temperature for several hours/days. Is the kit still OK?
- How do I locate and identify a Ureaplasma urealyticum colony?
CHANG MEDIUM® C, D, and In Situ are all evaluated on both primary and low passage amniocytes:
| CHANG MEDIUM® Formula | Description |
| CHANG MEDIUM® C |
|
| CHANG MEDIUM® In Situ |
|
| CHANG MEDIUM® D |
|
CHANG MEDIUM® MF and BMC are specifically formulated for hemopoietic cell culture:
| CHANG MEDIUM® Formula | Description |
| CHANG MEDIUM® BMC |
|
| CHANG MEDIUM® MF |
|
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How long can I store CHANG MEDIUM® once the supplement is added to the basal medium?
CHANG MEDIUM® C supplement may go through a single freeze-thaw cycle. The basal portion should not be frozen. Once mixed, the complete formula should be used within 10 days. CHANG MEDIUM® D and In Situ may undergo two freeze-thaw cycles and should be used within 14 days once opened. All open, complete formulas should be stored at 2-8° C.
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What is the proper way to reconstitute the CHANG MEDIUM® C lyophilized supplement?
Allow the supplement to equilibrate to room temperature. Using aseptic techniques, add the appropriate amount of sterile, distilled water to the lyophilizate. Swirl the vial gently. It may be necessary to incubate the vial at 37°C for 5 to 10 minutes to completely dissolve the supplement. Aseptically transfer the entire contents of the reconstituted CHANG MEDIUM® C supplement to the bottle of CHANG MEDIUM® B basal. Mix the complete CHANG MEDIUM® C well by swirling the bottle. Supplement aseptically with sterile L-glutamine, 10.0 mL/L. Antibiotics may be added if desired.
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What quality control is performed on CHANG MEDIUM® formulations?
All products manufactured at Irvine Scientific are subjected to rigorous Quality Control testing before they are released for sale. CHANG MEDIUM® products are tested for sterility, mycoplasma, pH, osmolality, and endotoxin. Function testing is performed as well, by an independent clinical testing lab, the Colorado Genetics Laboratory. Details on tests performed are available on the Certificate of Analysis which accompanies each shipment.
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What is "AmnioStat-FLM®-PG"?
The AmnioStat-FLM®-PG kit is an immunologic, qualitative agglutination test for screening phosphatidylglycerol, or PG, in human amniotic fluid. It is used to determine fetal lung maturity. The test requires less than 0.1 mL of specimen and can be performed in about 15 minutes.
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How is the AmnioStat-FLM®-PG kit configured?
Each AmnioStat-FLM®-PG kit comes with three levels of controls and agglutination cards. There are enough controls and cards to run a negative, low positive, and high positive with each patient. Materials needed, but not provided include disposable test tubes, test tube rack, micropipetters, disposable pipette tips, and a 60 rpm serological rotator. A low speed centrifuge may be needed.
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My AmnioStat-FLM®-PG kit was left at room temperature for several hours/days. Is the kit still OK?
The antibody in Reagent B is temperature-sensitive and should be stored only at 2-8° C. When the kit is received, it should be immediately placed in 2-8° C.
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How do I locate and identify a Ureaplasma urealyticum colony?
Ureaplasma urealyticum exhibits a colony morphology referred to as an "accretion" colony. The colony is small, 15 to 50 microns in diameter and appears as a brown, crystalline structure (without the application of Dienes stain.) This colony appearance is due to the formation of an ammonium precipitate around and throughout the colony. Begin by scanning the agar surface near the scratch made during sample inoculation and look for the small crystalline colonies. To definitively identify a ureaplasma colony, focus above and below the structure by varying the plane of focus and look for characteristic surface and sub-surface growth.
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