GAMETE, FERTILIZATION AND EMBRYO CULTURE MEDIA
All ProductsComplete Media
Culture Media
ECM/P-1
Modified Ham's F-10
Sequential Media
How long after opening bottles, can we use the products?
When stored as directed, products are stable until the expiration date shown on the bottle. Upon opening the bottles, it is recommended to use the product within 8 weeks.
^topWhat is the shelf life of Complete P-1®, Complete HTF and Complete Early Cleavage Medium® (ECM®) that is supplemented with protein?
The shelf life of the "Complete" range of products is 120 days from the date of manufacturing. Like all our media, the products will be stable and ready to use until the date shown on the bottles, as long as they are stored as directed. Once opened, it is recommended to use the media within 8 weeks.
^topIs it possible to add SSS to Complete P-1®?
Complete P-1® already contains 10% v/v of SSS - the typical concentration of SSS used for embryo culture. If higher concentrations of SSS are desired it is possible to add more SSS if needed as long as you consider that it already has 10% added.
^topWhat is the advantage of using the Early Cleavage Medium® (ECM®) over the Complete P-1®?
There is not necessarily an advantage of ECM® over P-1®. ECM® is our most recent formulation for pre-implantation embryos (cultured to D3 after fertilization), which has three additional components that are not present in P-1®, namely: glucose (0.5 mM), alanyl-glutamine (0.5 mM), and EDTA (10uM). P-1® does not have glucose or EDTA, and contains only one amino acid-Taurine (0.05 mM). ECM® also contains Taurine at the same concentration. We developed ECM® as an alternative to P-1® for those customers who preferred to have glucose in their first stage medium.
^topIs it okay to use Modified Ham's F-10 if it has been frozen?
No. As indicated in the product insert, Modified Ham's F-10 is stable and ready to use until the date shown on the bottle, as long as it is stored within 2°C to 8°C. The media should not be exposed to extreme temperatures at any time. If frozen, please do not use the product.
^topDo you carry sequential media without antibiotics?
No, all Irvine Scientific® Sequential Media contain Gentamicin-Antibiotic
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