To complement our media development and optimization services, Irvine Scientific offers Spent Media Analysis. This analysis provides an understanding of changes in culture conditions over time. We can provide data within approximately 10 business days.

Below are descriptions of our current offerings, as well as sample preparation instructions.

LEVEL ONE
Glucose, Lactate, Glutamine, Ammonia
(Nova BioAnalyzer)
These components are primary indicators of culture kinetics.
LEVEL TWO
Amino Acids
(HPLC with UV)
Includes 20 amino acids commonly found in cell culture media. Amino acids can be rate limiting factors for both growth and production.
LEVEL THREE
Water Soluble Vitamins
(HPLC with UV)
Includes pyridoxine, Niacinamide, Folic Acid, Vitamin B12, Riboflavin and Thiamine. This testing is primarily for in-depth media development.

Sample Preparation

• Spin down sample to remove cells
• Sterile filter through 0.2 micron filter
• Transfer at least 5mL of sample into a centrifuge tube 
• Label with company name, date, experiment name, and culture time point
• Freeze at -20C and send frozen (overnight with dry ice) to the attention of:
  David Ho, Industiral Cell Culture R&D (Analytical Testing)

For information on ordering Spent Media Analysis, please contact the Industrial Cell Culture technical department, at 1 (800) 437-5706 or 1 (949) 261-7800 or email us at tmrequest@irvinesci.com.