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CHANG | Cytogenetics
Does your CHANG Medium® contain FGFs (Fibroblast Growth Factors) or insulin?
Yes, all CHANG Medium® formulations contain both FGFs and insulin.
MSC SFM and XSFM | Cell Therapy
Has PRIME-XV MSC Expansion XSFM been used in expansion on microcarriers?
PRIME-XV MSC Expansion XSFM has been evaluated/proven for scale up productions using Corning/Hyperflask-CellBIND, data. Protocol and data are available…
T Cell CDM | Cell Therapy
Has PRIME-XV® T Cell CDM been tested on enriched cell populations? Th or Treg?
This media will support the expansion of enriched T cell populations. Currently, we have data that indicates that Th1…
T Cell CDM | Cell Therapy
How did we activate cells?
Coated cell culture plates or bags with anti-human CD3 and anti-human CD28 antibodies were used to activate the cells.…
Sperm Processing Media | Assisted Reproductive Technology
Sperm Washing Medium — How do I convert the rpm's to g-force?
Calculation formula: g = RCF (relative centrifugal force) = 0.00001118 × r × RPM, where g = Relative centrifuge…
Sperm Processing Media | Assisted Reproductive Technology
ISolate® — How do I obtain a 40% upper layer from your 50% upper layer?
To obtain a 40% upper layer from our 50% upper layer solution (99257), mix 8.0mL of (upper layer) 99275…
Sperm Processing Media | Assisted Reproductive Technology
ISolate® — How do I obtain an 80% lower layer from your 90% lower layer of ISolate®?
To obtain an 80% lower layer from our 90% lower layer solution (99258), mix 8.9mL of 99258 with 1.1mL…
T Cell CDM | Cell Therapy
How do we monitor changes in growth, since the media is devoid of Phenol red?
During development of the media, we tested cell viability and fold expansion of CD3+ (cell count) to monitor changes…
General | Industrial Cell Culture
How do we prevent mycoplasma contamination in cell culture?
Good aseptic techniques should be used in all laboratory applications. The best method for prevention is to test all…
Mycoplasma | Industrial Cell Culture
How do we prevent mycoplasma contamination in cell culture?
Good aseptic techniques should be used in all laboratory applications. The best method for prevention is to test all…