ISolate Concentrate

Catalog ID: 99306

Prepare custom density gradients to isolate desired quantities of motile sperm.

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ISolate Concentrate
99306-100 mL Login above or Register for pricing
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ISolate Concentrate (100%) provides full control over the density gradient sperm separation process. Large volume packaging allows for economical development of custom gradients that meet your laboratory’s procedures and needs. As one of the most cited density gradient sperm separation media, ISolate Concentrate is the ideal product for customizable sperm separation.

• ISolate can be diluted with mHTF or Multipurpose Handling Medium (MHM)
• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
• Quality tested by human sperm survival assay
• CE Marked

Quality Control Testing:

• Rabbit pyrogen test
• Sterility by the current USP, CFR and Ph. Eur.  Sterility Test
• Sperm Survival Assay
• pH
• Osmolality
• Endotoxin by Limulus Amebocyte Lysate (LAL)


Store at 2-8°C

Shelf Life

2 years from date of manufacture

Benedict SL et al. Performance of two commercially available density gradients for intrauterine insemination sperm preparation. Fertil Steril 2006; 86: S506-507

Cassuto NG et al. Correlation between DNA defect and sperm-head morphology. Reproductive Biomedicine Online 2013; 24: 211-218

Malvezzi et al. Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial.  Reproductive Biology and Endocrinology 2014; 12:121