ISolate® Stock Solution

Catalog ID: 99275

Single 90% gradient solution for rapid single layer separation of sperm or for creating custom two-layer gradient systems.

99275 - ISolate Stock Solution
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ISolate® Stock Solution
99275-100 mL Login above or Register for pricing

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ISolate Stock is a 90% density gradient medium used for single layer sperm separation or as the lower layer of a customized gradient developed by the end user based on the semen quality. As one of the most cited density gradient sperm separation media, ISolate Stock is the ideal medium for laboratories that prefer single layer separation or more control over their sperm yields.

• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
• Quality tested by human sperm survival assay
• ISolate can be diluted with mHTF or Multipurpose Handling Medium (MHM)
• Supplied in large economical packages
• CE Marked

Quality Control Testing:
• Rabbit pyrogen test
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• Sperm Survival Assay
• pH
• Osmolality

Storage

Store at 2-8°C

Shelf Life

2 years from date of manufacture

Benedict SL et al. Performance of two commercially available density gradients for intrauterine insemination sperm preparation. Fertil Steril 2006; 86: S506-507

Cassuto NG et al. Correlation between DNA defect and sperm-head morphology. Reproductive Biomedicine Online 2013; 24: 211-218

Malvezzi et al. Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial.  Reproductive Biology and Endocrinology 2014; 12:121