Catalog ID: 99264

Sperm separation kit designed to quickly produce clean samples, with high concentrations of progressive motile sperm from both healthy and low quality semen.

99264 - ISolate
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99264-12X6 mL Login above or Register for pricing
99264-2X50 mL    
99264-2x100 mL    
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As one of the most cited density gradient sperm separation media, ISolate is the media of choice for laboratories worldwide to produce clean, robust samples with optimal recovery rates. It is supplied ready-to-use as a dual density gradient solution to process the wide variety of semen samples encountered in the laboratory. The kit, which consists of a 50% upper layer and 90% lower layer, support use with a two layer protocol for healthy samples or with a single layer protocol that enables separation of motile sperm from low quality semen samples. ISolate effectively removes cellular contaminants (dead sperm, white cells, and miscellaneous debris) to yield a sample that contains predominantly progressive, motile sperm, capable of fertilizing the oocyte for IUI, IVF and ICSI.

• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
• Highest quality raw materials
• Quality tested by human sperm survival assay
• 90% stock and 100% concentrate available for custom dilutions
• CE Marked

Quality Control Testing:

• Rabbit pyrogen test
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• Sperm Survival Assay
• pH
• Osmolality
• Endotoxin by Limulus Amebocyte Lysate (LAL)

Case Study: Focus on Sperm to Achieve Better Pregnancy Rates

A case study conducted by Dr. Mehta, Lab Director at Queen’s Hospital in Romford, UK, showed a 10% increase in pregnancy rate using ISolate and Multipurpose Handling Medium compared to leading competitor's media.

See full case study



Store at 2-8°C

Shelf Life

2 years from date of manufacture

Benedict SL et al. Performance of two commercially available density gradients for intrauterine insemination sperm preparation. Fertil Steril 2006; 86: S506-507

Cassuto NG et al. Correlation between DNA defect and sperm-head morphology. Reproductive Biomedicine Online 2013; 24: 211-218

Malvezzi et al. Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial.  Reproductive Biology and Endocrinology 2014; 12:121