BalanCD HEK 293 Feed

Catalog ID: 91166

Enhance post-transfection growth and protein production of multiple HEk293 cell lines

91166 BalanCD HEK 293 Feed
Login above or Register for pricing
BalanCD HEK 293 Feed
91166-500 mL Login above or Register for pricing
Request More Information

In order to purchase products you must first fill out a customer application. Once your application has been approved you will be able to purchase products directly.

Please upgrade your account today!

BalanCD HEK293 Feed is formulated to significantly increase protein yields in suspension-adapted HEK293 cell lines post-transfection.

  • Increase viable cell density and culture duration to maximize yield
  • Use the same Feed for a wide range of HEK293 cell lines
  • Fulfill regulatory requirements
    • Chemically-defined, animal component-free formula
    • Drug Master File (DMF) submitted to US FDA

Two-fold increase in titer compared to other commercially-available media


HEK293-6E cells were cultured in duplicate 125mL Erlenmeyer flasks with a 30 mL working volume. Cells were seeded at 3 x 105 cells/mL in BalanCD HEK 293 medium. PEI-mediated transfection was performed on day 3 to produce a biosimilar antibody. BalanCD HEK293 medium was supplemented with a 5% v/v addition of BalanCD HEK293 Feed on days 1-4 post-transfection.  Protein titer was measured on days 7, 9, and 11 post-transfection. Cultures terminated when viability dropped below 70%, (data not shown). Data shown represents the average of two duplicate cultures.


Shelf Life

12 months from the date of manufacture (DOM)