PRIME-XV Dendritic Cell Maturation CDM
PRIME-XV Dendritic Cell Maturation CDM is a chemically-defined, animal component-free medium developed for the in vitro differentiation of monocytes into immature dendritic cells (iDCs) and subsequent maturation to produce mature dendritic cells (mDCs). Free of the undefined components found in animal- or human-derived raw materials that affect functional capacity PRIME-XV Dendritic Cell Maturation CDM supports effective maturation of mDCs with the functional capacity to induce T cell response.
Formulated to produce high yields of functional cells
- Achieve high yields of DCs with the desired characteristics and functionality
- Maintains potency of dendritic cells for T cell stimulation
- Ready-to-use, just supplement with desired cytokine cocktails
Designed and manufactured to facilitate transfer from research to clinic
- Free from any animal or human-derived components
- Manufactured in compliance with cGMP regulations
- Robust raw material controls and supply chain management
- Traceablity documentation provided including Certificates of Analysis, Certificates of Origin and a Drug Master File (DMF) filed with the US FDA*
*DMF filing in process
This product is shipped at 2-8 °C. Upon receipt, store immediately at 2-8 °C and protect from light.
Handle using aseptic techniques to avoid contamination. Unopened liquid medium is stable for 14 months from date of manufacture, as indicated on the label, when stored at 2-8 °C and protected from light.
Figure 1. Achieve high yields of viable cells in chemically-defined culture conditions.
Negatively enriched CD14+ monocytes from 5 different donors were cultured in either PRIME-XV Dendritic Cell Maturation CDM, other commercially available medium, or RMPI +10% FBS for 6 days in the presence of GM-CSF and IL-4 to induce differentiation, then subsequently cultured for 2 more days in TNF-α, IL-1β, IL-6, and PGE2 to induce maturation for a combined culture duration of 8 days in each respective medium. Resulting cells were analyzed by flow cytometry to measure viable cell density. Yield shown as total viable cells as a percentage of initial CD14+ monocytes plated on day 0.
Figure 2. Functionality demonstrated by surface marker expression profile.
Negatively selected CD14+ monocytes were cultured in PRIME-XV Dendritic Cell Maturation CDM for 6 days in the presence of GM-CSF and IL-4 to induce differentiation, and subsequently cultured for 2 more days in the Jonuletiy cocktail (TNF-α, IL-1β, IL-6, and PGE2) to induce maturation for a combined culture duration of 8 days. The cells were harvested and analyzed by flow cytometry for surface marker expression. Cells cultured with the Jonuleit cocktail were analyzed at day 0 (monocyte), day 6 (immature DC), and day 8 (mature DC) and demonstrated typical surface marker expression profiles throughout DC maturation.
For more data download the Data Sheet.