Vit Kit - Warm NX

Catalog ID: 90183

Warm oocytes and embryos in a modern vitrification system.

Vit Kit-NX
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Vit Kit - Warm NX
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Vit Kit - Warm NX is an adaptable, cost-effective system for use in the thawing of oocytes, pronuclear zygotes, cleavage stage embryos, and blastocyst stage embryos. Unlike many vitrification kits that feature a mono-buffered system and M199 base medium originally formulated for chick embryo fibroblasts, Vit Kit - Warm NX is derived from current IVF media tailored specifically for human embryo culture.

The backbone of Vit Kit - Warm NX is Continuous Single Culture medium, an IVF medium intended for conventional fertilization and culture from Day 1 and beyond. Similar to our Multipurpose Handling Medium, Vit Kit-NX takes advantage of two buffers, HEPES and MOPS, to provide a more secure pH environment for oocytes and embryos during vitrification. The integration of these key ingredients delivers consistent results, including high oocyte and embryo survival rates, while also offering an effective, flexible, and economical media ideally suited for the nuanced needs of the modern IVF laboratory.

  • Formula based on Continuous Single Culture medium (CSCM) and Multipurpose Handling Medium (MHM) to minimize stress on oocytes and embryos, and achieve high survival rates
  • Dual-buffered (HEPES and MOPS) to provide a more secure pH environment
  • Trehalose as the non-permeating cryoprotectant supports higher glass transition temperature and membrane stabilization than sucrose
  • Supplemented with 20% (v/v) Dextran Serum Supplement (DSS) for a final concentration of 10 mg/mL HSA and 4 mg/mL Dextran
  • Contains no phenol red

Each kit can be used for up to 12 warming applications with embryos or oocytes.

Versatile Formulas Deliver Consistent Survival Rates Across Different Vitrification Media

Vit Kit - Warm NX was capable of warming human oocytes frozen in Vit Kit (A) and a top competitor's vitrification media (B).

Previously frozen MII oocytes were re-frozen in different vitrification media with the Cryolock device. MII oocytes were counted towards the survival rate and were recovered in CSCM-NXC (PN 90168) for the immediate and 2h post-thaw time points. Study was performed in collaboration with World Egg Bank in Phoenix, Arizona, USA.

Storage

Store at 2-8°C

Shelf Life

6 months from date of manufacture