Freezing Medium - TYB with Glycerol & Gentamicin

Catalog ID: 90128

Sperm cryopreservation medium containing TEST-yolk buffer to attain high postthaw sperm recovery.

Freezing Medium TYB – sperm cryopreservation media
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Freezing Medium - TYB with Glycerol & Gentamicin
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90128-20X5 mL    
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Freezing Medium TYB contains TEST-yolk buffer (TYB) to recover more motile sperm post-thaw than glycerol-only media (Hallak 2000, Nallella 2004). TEST-yolk buffer is a semen extender and cryobuffer that can be paired with glycerol to achieve high sperm motility and viability. The egg yolk is from USDA certified virus-free flocks and washed from the sperm after cryopreservation. Freezing Medium TYB is also the only CE-marked and FDA-approved sperm freezing media containing TYB and glycerol. As one of the most referenced sperm cryopreservation media worldwide, Freezing Medium TYB has established itself as the standard for performance.

• 2 year shelf life

• Contains:
  - 20% egg yolk from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56°C for 30 minutes
  - 12% glycerol
  - 10µg/mL gentamicin sulfate

• CE Marked

Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sperm Cryosurvival assay
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test
• pH
• Osmolality


Store Below -10°C

Shelf Life

2 years from date of manufacture

Hallak J et al. Cryopreservation of Human Spermatozoa: Comparison of TEST-Yolk Buffer and Glycerol. Int J Fertil 2000; 45(1): 38-42

Bhattacharya J et al. A comparative study on TEST-yolk buffer and human sperm preservation medium on post thaw characteristics of human sperm from pre-freeze specimens. Fertil Steril 2006; 86:S200

Hammadeh M et al. Comparison between human sperm preservation medium and TEST-yolk buffer on protecting chromatin and morphology integrity of human spermatozoa in fertile and subfertile men after freeze-thawing procedure. J Androl 2001; 22(6): 1012-1018

Nallella et al. Cryopreservation of human spermatozoa: Comparison of two cryopreservation methods and three cryoprotectants. Fertil Steril 2004; 82:913-918