Serum-Free or Chemically-Defined Media

Serum-free and chemically-defined media offer customers better lot-to-lot consistency because they contain fewer undefined components, such as serum. Chemically-defined media contains components that are all known.

Chinese Hamster Ovary (CHO) cells are used in the study of genetics, nutrition, and gene expression, and are the most commonly used mammalian host for commercial production of recombinant protein therapeutics.

Serum-free and chemically-defined media are lower in protein content than media supplemented with serum, which can simplify the purification process and increase the yield of the end product. These products are free of animal-derived components, and only contain known chemical components.

Hybridoma cells are a hybrid of a lymphocyte (usually a B-Cell) and a myeloma cancer cell. These cells are useful since they can produce monoclonal antibodies as opposed to polyclonal antibodies.

Human Embryonic Kidney cells (HEK 293) are a commonly-used cell line for adenovirus expression and recombinant protein production.

Insect cell lines, such as Sf9 and Sf21, support Baculovirus Expression Vector Systems (BEVS) and are frequently used for protein expression, including recombinant proteins and viral-like particles, as well as viral vector production.