Serum-Free or Chemically-Defined Media

Serum-free and chemically-defined media offer customers better lot-to-lot consistency because they contain fewer undefined components, such as serum. Chemically-defined media contains components that are all known.

Maximizing the productivity, growth, and viability of CHO cell lines is an integral part of the development and commercialization of biotherapeutics. To help ensure this, the BalanCD CHO Media Platform delivers a scalable platform of chemically-defined, animal component-free growth and feed media that foster consistent growth and production at all phases:

Discovery Transfectory Delivers milligram- to gram-scale yield of recombinant proteins using a simplified and affordable protocol
Cell Line Development Growth A and Media Survey Panel Delivers milligram- to gram-scale yield of recombinant proteins using a simplified and affordable protocol
Process Development & Scale-up Growth A and Custom Media Services Delivers higher protein titer and formulated to support full-scale commercial manufacturing
cGMP Manufacturing Feed 4 Boost manufacturing outcomes with the growth and performance benefits of our most advanced, high-nutrient feed medium


Purpose-built BalanCD CHO growth and feed media are formulated for industrial applications and for a smooth transition from early stage into commercial bioproduction. Scalability to a commercial batch size is ensured using a Rational Culture Media Design approach of sequential, complementary development methods to leverage our scientists’ knowledge of the effects of components and process parameters.

Designed to nurture and advance every step of development, the BalanCD CHO Media Platform provides reliable, consistent results from discovery to commercialization.


Serum-free and chemically-defined media are lower in protein content than media supplemented with serum, which can simplify the purification process and increase the yield of the end product. These media are free of animal-derived components, and only contain known chemical components.


Hybridoma cells are a hybrid of a lymphocyte (usually a B-Cell) and a myeloma cancer cell. These cells are useful since they can produce monoclonal antibodies as opposed to polyclonal antibodies.


The BalanCD HEK293 scalable system from FUJIFILM Irvine Scientific provides the optimal balance between growth and production to maximize productivity from mammalian cell cultures. Comprised of chemically-defined, animal component-free BalanCD HEK293 medium and BalanCD HEK293 Feed, this versatile system supports a range of applications including production of viral vectors for gene therapy, transient protein expression, and recombinant protein production.

  • Chemically-defined, animal component-free
  • Rapid, scalable production of viral vectors and proteins for bioproduction
  • Supports use for a wide range of HEK293 cell lines
  • Increases productivity in transient protein expression
  • For use in flasks, spinner flasks, and bioreactors

BalanCD HEK293 Medium. A versatile, chemically-defined, animal component-free medium optimized for transfection and production of viral vectors and recombinant proteins.

  • Rapid, scalable production of viral vectors and proteins using the same medium for seeding, transfection, and production
  • Achieve optimal production yields within 48 hours
  • Achieve higher cell densities while maintaining viability
  • Drug Master File (DMF) filed with US FDA

BalanCD HEK293 Feed. Formulated to maximize protein yields in suspension-adapted HEK293 cell lines post-transfection.

  • Increases culture longevity and maximizes growth

Anti-Clumping Supplement. Animal component-free and enzyme-free general use reagent designed to reduce cell aggregation for various cell types in suspension culture.

Contact a media specialist today to learn more about the BalanCD HEK293 system.


Insect cell lines, such as Sf9 and Sf21, support Baculovirus Expression Vector Systems (BEVS) and are frequently used for protein expression, including recombinant proteins and viral-like particles, as well as viral vector production.