International Society for Stem Cell Research (ISSCR) 2016 Annual Meeting
Posted in Cell Therapy
San Francisco, CA | Jun 22, 2016–Jun 25, 2016
Join us for the Innovation Showcase
Thursday, 23rd June
11:30am – 12:30pm
Moscone West, Level 2, room 2002
Optimizing Culture Medium That Maintains Mouse Hematopoietic Stem Cells
Dr. Hideyuki Oguro,
Department of Pediatrics, University of Texas Southwestern Medical Center
Hematopoietic stem cell expansion in culture is a critical yet challenging goal to achieve to facilitate gene therapy and increase the transplantation safety and efficacy. In this workshop, we will share the evaluation results of culturing 20 isolated, CD150+CD48-/lowCD34-/lowLineage-Sca-1+c-kit+ highly enriched HSCs from young-adult mouse bone marrow in prototypes media comparing to the commonly used SF-O3, supplemented with SCF and TPO. Data from colony assay and transplantation studies demonstrated a simplified, ready-to-use medium that could allow us to continue fine tuning the culture components to further improve HSC maintenance ex vivo.
Impacts of Undefined Components for Primary Cell Cultures
Jessie H.-T. Ni, Ph.D.
Chief Scientific Officer, Irvine Scientific
Natures of stem/progenitor cells generated from ex vivo cultures are critically dependent on the quality of supplemented serum or serum-replacement components, e.g. cytokines or lipids. We will demonstrate through historical data and case studies, how these “undefined” components impact the culture performance of major adult stem cells and immune cells. Potential solutions will be recommended that could allow researchers to best achieve consistent and desired ex vivo expansions of targeted stem/primary cells under less-defined culture conditions.
Difference in Immunomodulation and Multipotent Potential Exists Among Human Adipose Tissue-, Bone Marrow-, and Umbilical Cord-Derived Mesenchymal Stem/Stromal Cells
Dr. Jessie H.-T. Ni, CSO, Irvine Scientific
Friday, June 24,
7:00pm to 8:00pm
Poster #: F1028, within the ‘Mesenchymal Stem Cell Differentiation’ section at Session III
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