What is the proper way to reconstitute the CHANG Medium C lyophilized supplement?
First allow the supplement to equilibrate to room temperature. Once equilibrated, under aseptic conditions the appropriate amount of sterile, distilled water can be added to the lyophilizate. Swirl the vial gently. It may be necessary to incubate the vial at 37°C for 5 to 10 minutes to completely dissolve the supplement. Aseptically transfer the entire contents of the reconstituted CHANG Medium C supplement to the bottle of CHANG Medium B basal. Mix the complete CHANG Medium C well by swirling the bottle. Supplement aseptically with sterile L-glutamine, 10.0 mL/L. Antibiotics may be added if desired.