Cell Culture Media

Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components.

Ham’s F-10 and F-12 media are standard for cell culture. Originally developed for use in cloning Chinese Hamster Ovary (CHO) and lung cells, these media are useful with serum supplements for a variety of cell culture applications, including the growth of myeloma and hybridoma cells.

McCoy’s Medium 5A is for primary and established cell lines. It contains increased glucose, additional vitamins, high phosphate, and peptone. Click’s Medium is a modification of Eagle’s Essential Medium with Hanks’ Salts (HMEM) and contains higher concentrations of essential amino acids, in addition to non-essential amino acids, sodium pyruvate, and nucleic acid precursors.

Minimum Essential Medium (MEM) was developed by Harry Eagle and is one of the most widely used of all synthetic cell culture media. It is designed for a wide range of species and cells. Alpha MEM is a modified Eagle’s MEM designed for growth of a mouse-hamster-hybridoma cell line.