PRIME-XV® Osteogenic Differentiation Serum-Free Medium is manufactured in a GMP environment and made to induce optimal osteogenesis of human mesenchymal stem cells. Osteogenesis in PRIME-XV Osteogenic Differentiation SFM was validated by calcium deposition and expression of RUNX2 and OSTEOCALCIN.

Mesenchymal stem/ stromal cells (MSCs) are an important source of cells for maintaining bone homeostasis and bone tissue engineering, partly by their ability to differentiate into osteocytes. While original research on osteocytes suggests that these cells play a passive role, numerous recent studies have found osteocytes to be responsible for modulating osteoclast and osteoblast activities.  Efficient osteogenic differentiation is marked by positive alkaline phosphatase activity, an increase in calcium deposition into the extracellular matrix, and expression of RUNX2 and OSTEOCALCIN by osteocytes.