Is adaptation required when transitioning from a serum-containing media to PRIME-XV NK Cell CDM?
In the case of PBMC-derived NK cell expansion, cells were thawed and directly expanded into PRIME XV NK Cell CDM. No adaptation to serum-free conditions was necessary.
NK92 used had been expanded in media containing 10% FBS prior to experiments with PRIME XV NK Cell CDM. No sequential adaptation into lower FBS percentage was required.
However, if you experience an increase in doubling time ,or any impact in cell culture parameters in your setup, please consider a sequential adaptation into lower serum-containing conditions before fully transitioning into PRIME XV NK Cell CDM. This is particularly relevant if cells have been grown in serum-containing conditions for multiple passages prior to use of chemically-defined media.