How often are MSCs passaged when cultured in PRIME-XV® MSC Expansion SFM?
Cells are often passaged when they reach 80% confluency, or the coverage of the culture vessel by the cells. One hundred percent confluency refers to cells covering 100% of the cultured surface area. Time between each passage depends on the media performance and initial seeding density. However, on average, human mesenchymal stem cells (MSCs) cultured in PRIME-XV MSC EXPANSION SFM is passaged every 3-4 days.