Can I use the PRIME-XV® MSC Expansion SFM to culture cells without a coating substrate?


No, it is not recommended. Unlike serum-containing MSC culture medium, serum-free culture condition for human MSCs requires fibronectin (such as PRIME-XV Human Fibronectin, Catalog # 31002) or our proprietary matrix protein (PRIME-XV MatrIS F, Catalog # 31001). Without a coating substrate, MSCs will die in absence of serum.