Can I use the PRIME-XV MSC Expansion SFM to culture cells without a coating substrate?


It is highly recommended to use MSC expansion medium with a coating substrate. In house experiments demonstrated higher success rate with fibronectin or MatrIS F. Unlike serum-containing MSC culture medium, serum-free culture condition for human MSCs requires fibronectin (such as PRIME-XV Human Fibronectin) or our proprietary matrix protein (PRIME-XV MatrIS F).