Are the original T cell fractions driven towards a specific T cell phenotype upon expansion? Are the CD4+ and CD8+ fractions maintained in similar ratios after expansion?
The ratio of CD4+ to CD8+ cells can be affected by a number of factors, including the donor from which there were derived, the presence of monocyte populations, the method of activation, etc. As a result, the CD4/CD8 ratio may be affected during or after expansion. While the ratio may change during culture, our T Cell CDM can support the expansion of both populations. Multiple donors were used in the development of the media.