FAQ

Q.

Why is EDTA added to your culture media?

A.

The addition of EDTA has been shown to enhance the rate of early cleavage in embryos in culture and also serves as an antioxidant to stabilize the media.


 

References

  1. Berthois, Y., Katzenellenbogen, J. A., & Katzenellenbogen, B. S. (1986). Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proceedings of the National Academy of Sciences,83(8), 2496-2500. doi:10.1073/pnas.83.8.2496
  2. Guerif, F., Mckeegan, P., Leese, H. J., & Sturmey, R. G. (2013). A Simple Approach for COnsumption and RElease (CORE) Analysis of Metabolic Activity in Single Mammalian Embryos. PLoS ONE,8(8). doi:10.1371/journal.pone.0067834
  3. Zander-Fox, D. & Lane, M. The future of human embryo culture media – or have we reached the ceiling? in The Human Embryo (ed. Yamada, S. & Takakuwa, T.) 73-98 (Intech, 2012).