FAQ

Q.

What is the recommended amount of protein supplementation to be used with Continuous Single Culture® (CSCM) and Continuous Single Culture®–NX (CSCM-NX)?

A.

When using Irvine Scientific HSA, a 100 mg/mL protein solution, we recommend 5% v/v for a final concentration of 5 mg/mL. For example, to prepare 10 mL of medium, add 0.5 mL of HSA solution to 9.5 mL of the medium. When using Irvine Scientific SSS™, a 60 mg/mL protein solution, we recommend 10% v/v for a final concentration of 6 mg/mL. For example, to prepare 10 mL of medium, add 1.0 mL SSS™ to 9.0 mL of medium.